modified_cellulolytic_haloarchaea_medium

Overview

External ontology

mediadive.medium:J1181 (source link unavailable) — MODIFIED CELLULOLYTIC HALOARCHAEA MEDIUM

Applications

• Microbial cultivation

pH

7.0

High-metal flag

True

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1181

Composition

flowchart LR
    CultureMech_002353["modified_cellulolytic_haloarchaea_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002353 --> ing0_Yeast_extract
    ing1_NH4Cl["NH4Cl"]
    CultureMech_002353 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_Cellobiose["Cellobiose"]
    CultureMech_002353 --> ing2_Cellobiose
    CHEBI_17057["CHEBI:17057"]
    ing2_Cellobiose -.-> CHEBI_17057
    ing3_NaCl["NaCl"]
    CultureMech_002353 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002353 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002353 --> ing5_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing6_KCl["KCl"]
    CultureMech_002353 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_CaCl2["CaCl2"]
    CultureMech_002353 --> ing7_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing7_CaCl2 -.-> CHEBI_3312
    ing8_EDTA["EDTA"]
    CultureMech_002353 --> ing8_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing8_EDTA -.-> CHEBI_4735
    ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002353 --> ing9_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002353 --> ing10_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002353 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_H3BO3["H3BO3"]
    CultureMech_002353 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002353 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002353 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002353 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002353 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_K2HPO4["K2HPO4"]
    CultureMech_002353 --> ing17_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing17_K2HPO4 -.-> CHEBI_131527
    ing18_KH2PO4["KH2PO4"]
    CultureMech_002353 --> ing18_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing18_KH2PO4 -.-> CHEBI_63036
    ing19_Biotin["Biotin"]
    CultureMech_002353 --> ing19_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing19_Biotin -.-> CHEBI_15956
    ing20_Folic_acid["Folic acid"]
    CultureMech_002353 --> ing20_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing20_Folic_acid -.-> CHEBI_27470
    ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002353 --> ing21_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing22_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002353 --> ing22_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing22_Thiamine_HCl -.-> CHEBI_49105
    ing23_Riboflavin["Riboflavin"]
    CultureMech_002353 --> ing23_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing23_Riboflavin -.-> CHEBI_17015
    ing24_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002353 --> ing24_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing24_Nicotinic_acid -.-> CHEBI_15940
    ing25_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002353 --> ing25_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing25_Calcium_pantothenate -.-> CHEBI_31345
    ing26_Vitamin_B12["Vitamin B12"]
    CultureMech_002353 --> ing26_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing26_Vitamin_B12 -.-> CHEBI_176843
    ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002353 --> ing27_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing28_Lipoic_acid["Lipoic acid"]
    CultureMech_002353 --> ing28_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing28_Lipoic_acid -.-> CHEBI_16494

Ingredients (29)

Preparation

1. Add components and bring volume to 958 ml with distilled water. For preparation of solid medium, add 20 g/L agar (if necessary, use washed agar: see Medium No. 574). Autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized) to the medium.
2. Readjust pH to 7.0 with a sterilized 10% Na2CO3 solution, if necessary.
3. Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.5 with 1 M Tris base.
4. Adjust pH to 3.6.
5. Combine the two solutions and check that pH is close to 7.5. Add an equal volume of distilled water.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• modified_cellulolytic_haloarchaea_medium (CultureMech:002353)