Overview
- External ontology
- mediadive.medium:J1183 (source link unavailable) — CHLOROFLEXUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1183
Composition
flowchart LR
CultureMech_002354["chloroflexus_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002354 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NH4Cl["NH4Cl"]
CultureMech_002354 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002354 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_KCl["KCl"]
CultureMech_002354 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NaCl["NaCl"]
CultureMech_002354 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_002354 --> ing5_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing5_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing6_Yeast_extract["Yeast extract"]
CultureMech_002354 --> ing6_Yeast_extract
ing7_HEPES["HEPES"]
CultureMech_002354 --> ing7_HEPES
CHEBI_46756["CHEBI:46756"]
ing7_HEPES -.-> CHEBI_46756
ing8_Biotin["Biotin"]
CultureMech_002354 --> ing8_Biotin
CHEBI_15956["CHEBI:15956"]
ing8_Biotin -.-> CHEBI_15956
ing9_Folic_acid["Folic acid"]
CultureMech_002354 --> ing9_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing9_Folic_acid -.-> CHEBI_27470
ing10_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002354 --> ing10_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing10_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing11_Thiamine_HCl["Thiamine HCl"]
CultureMech_002354 --> ing11_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing11_Thiamine_HCl -.-> CHEBI_49105
ing12_Riboflavin["Riboflavin"]
CultureMech_002354 --> ing12_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing12_Riboflavin -.-> CHEBI_17015
ing13_Nicotinic_acid["Nicotinic acid"]
CultureMech_002354 --> ing13_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing13_Nicotinic_acid -.-> CHEBI_15940
ing14_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
CultureMech_002354 --> ing14_DL_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing14_DL_Calcium_pantothenate -.-> CHEBI_31345
ing15_Vitamin_B12["Vitamin B12"]
CultureMech_002354 --> ing15_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing15_Vitamin_B12 -.-> CHEBI_176843
ing16_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002354 --> ing16_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing16_p_Aminobenzoic_acid -.-> CHEBI_30753
ing17_EDTA["EDTA"]
CultureMech_002354 --> ing17_EDTA
CHEBI_4735["CHEBI:4735"]
ing17_EDTA -.-> CHEBI_4735
ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002354 --> ing18_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002354 --> ing19_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing20_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002354 --> ing20_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing20_MnCl2_x_4_H2O -.-> CHEBI_86368
ing21_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002354 --> ing21_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing21_CoCl2_x_6_H2O -.-> CHEBI_53503
ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002354 --> ing22_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002354 --> ing23_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing24_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002354 --> ing24_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing24_CuCl2_x_2_H2O -.-> CHEBI_86318
ing25_H3BO3["H3BO3"]
CultureMech_002354 --> ing25_H3BO3
CHEBI_33118["CHEBI:33118"]
ing25_H3BO3 -.-> CHEBI_33118
Ingredients (26)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.499002 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.499002 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.299401 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.499002 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
0.499002 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
0.0998004 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 7 | Yeast extract | 0.998004 G_PER_L | ||
| 8 | HEPES | 2.99401 G_PER_L |
CHEBI:46756
HEPES
|
|
| 9 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
|
| 10 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
|
| 11 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 12 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 13 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 14 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.05 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 15 |
DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 16 | Vitamin B12 | 0.005 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 17 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 18 |
EDTA
C10H16N2O8 292.244 g/mol
|
5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 19 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 20 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 21 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 22 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 23 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 24 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 25 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 26 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
Preparation
- After autoclaving, add 1 ml of 5% Na2S x 9H2O solution (autoclaved), 1 ml of 5% CaCl2 x 2H2O solution (autoclaved) and 10 ml of 3% NaHCO3 solution (filter-sterilized). Adjust pH to 7.5.
- Adjust pH to 3 with HCl.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- chloroflexus_medium (CultureMech:002354)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.932804Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1183 |
| 2026-03-13T06:19:12.644511Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002354 |
| 2026-03-17T06:30:42.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.653490+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CHLOROFLEXUS MEDIUM" to "chloroflexus_medium" for consistent matching |
| 2026-06-05T07:40:53.323944+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.480154+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:27.464501+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e1e4e80ee4bfe8a2964f4c45a7fcaf5beb2d9a865f26e731cfcd75fd878c8b08 |