atalassotoga_azc2201_medium

CultureMech:002355 bacterial COMPLEX LIQUID
Original name: ATALASSOTOGA AZC2201 MEDIUM

Overview

External ontology
mediadive.medium:J1184 (source link unavailable) — ATALASSOTOGA AZC2201 MEDIUM
Applications
  • Microbial cultivation
pH
4.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1184

Composition

flowchart LR
    CultureMech_002355["atalassotoga_azc2201_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002355 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002355 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002355 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_002355 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002355 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_K2HPO4["K2HPO4"]
    CultureMech_002355 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_KH2PO4["KH2PO4"]
    CultureMech_002355 --> ing6_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing6_KH2PO4 -.-> CHEBI_63036
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002355 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002355 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_Resazurin["Resazurin"]
    CultureMech_002355 --> ing9_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Resazurin -.-> CHEBI_8806
    ing10_Yeast_extract["Yeast extract"]
    CultureMech_002355 --> ing10_Yeast_extract
    ing11_Fructose["Fructose"]
    CultureMech_002355 --> ing11_Fructose
    CHEBI_28757["CHEBI:28757"]
    ing11_Fructose -.-> CHEBI_28757
    ing12_Na2S2O3["Na2S2O3"]
    CultureMech_002355 --> ing12_Na2S2O3
    CHEBI_132112["CHEBI:132112"]
    ing12_Na2S2O3 -.-> CHEBI_132112
    ing13_HCl["HCl"]
    CultureMech_002355 --> ing13_HCl
    CHEBI_17883["CHEBI:17883"]
    ing13_HCl -.-> CHEBI_17883
    ing14_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002355 --> ing14_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing14_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing15_ZnCl2["ZnCl2"]
    CultureMech_002355 --> ing15_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing15_ZnCl2 -.-> CHEBI_49976
    ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002355 --> ing16_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing17_H3BO3["H3BO3"]
    CultureMech_002355 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002355 --> ing18_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing19_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002355 --> ing19_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing19_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002355 --> ing20_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002355 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing22_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002355 --> ing22_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing22_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing23_NiSO4_x_7_H2O["NiSO4 x 7 H2O"]
    CultureMech_002355 --> ing23_NiSO4_x_7_H2O
    CHEBI_53504["CHEBI:53504"]
    ing23_NiSO4_x_7_H2O -.-> CHEBI_53504
    ing24_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002355 --> ing24_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing24_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing25_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002355 --> ing25_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing25_Na2SeO3_x_5_H2O -.-> CHEBI_131361

Ingredients (26)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
0.957854 G_PER_L CHEBI:26710
sodium chloride
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.957854 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.478927 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 KCl
Cl.K 74.551 g/mol
0.287356 G_PER_L CHEBI:32588
potassium chloride
5 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.287356 G_PER_L CHEBI:62946
ammonium sulfate
6 K2HPO4
HO4P.2K 174.174 g/mol
0.0191571 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
7 KH2PO4
H2O4P.K 136.084 g/mol
0.0191571 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
8 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.191571 G_PER_L CHEBI:86158
calcium chloride dihydrate
9 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.287356 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
10 Resazurin
C12H7NO4 229.191 g/mol
0.000957854 G_PER_L CHEBI:8806
Resazurin
11 Yeast extract 10 G_PER_L
12 Fructose 20 G_PER_L CHEBI:28757
fructose
13 Na2S2O3
2Na.O3S2 158.111 g/mol
10 G_PER_L CHEBI:132112
sodium thiosulfate
14 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
15 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
16 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
17 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
18 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
19 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
20 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
21 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate
23 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1.42 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
24 NiSO4 x 7 H2O
7H2O.Ni.O4S 280.861 g/mol
1.6 G_PER_L CHEBI:53504
nickel sulfate heptahydrate
25 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.038 G_PER_L CHEBI:63939
sodium tungstate dihydrate
26 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.003 G_PER_L CHEBI:131361
disodium selenite pentahydrate

Preparation

  1. Mix components thoroughly and adjust pH to 4.0. Bring to a boil and cool under a N2 gas stream. Distribute the medium into culture vessels (e.g., 10 ml of the medium in the Hungate tubes) under a N2 atmosphere, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized and stored under a N2 atmosphere):

Solutions

Vitamin solution

Dose: 1 G_PER_L

See mediadive_6241_Vitamin_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:18.936852Z mediadive-import Imported from MediaDive Source: JCM, ID: J1184
2026-03-09T08:04:39.010410+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:12.656659Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002355
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:15.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.668070+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ATALASSOTOGA AZC2201 MEDIUM" to "atalassotoga_azc2201_medium" for consistent matching
2026-05-16T09:03:25.129608+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:50.016874+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.554616+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:53.623828+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:24.393166+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 2c8a8ada2d946d0caed8e5b78b7d588117d93beac667a20e7fbfacc534941fa1