acidihalobacter_prosperus_f5_medium

CultureMech:002364 bacterial DEFINED LIQUID
Original name: ACIDIHALOBACTER PROSPERUS F5 MEDIUM

Overview

External ontology
mediadive.medium:J1197 (source link unavailable) — ACIDIHALOBACTER PROSPERUS F5 MEDIUM
Applications
  • Microbial cultivation
pH
2.5
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1197

Composition

flowchart LR
    CultureMech_002364["acidihalobacter_prosperus_f5_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002364 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002364 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002364 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_K2HPO4["K2HPO4"]
    CultureMech_002364 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002364 --> ing4_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing5_K2S4O6["K2S4O6"]
    CultureMech_002364 --> ing5_K2S4O6
    CHEBI_86466["CHEBI:86466"]
    ing5_K2S4O6 -.-> CHEBI_86466
    ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002364 --> ing6_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing7_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002364 --> ing7_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing7_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing8_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002364 --> ing8_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing8_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing9_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002364 --> ing9_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing9_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing10_Cr2_SO4_3_x_n_H2O["Cr2(SO4)3 x n H2O"]
    CultureMech_002364 --> ing10_Cr2_SO4_3_x_n_H2O
    CHEBI_53471["CHEBI:53471"]
    ing10_Cr2_SO4_3_x_n_H2O -.-> CHEBI_53471
    ing11_H3BO3["H3BO3"]
    CultureMech_002364 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002364 --> ing12_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing13_NiSO4_x_6_H2O["NiSO4 x 6 H2O"]
    CultureMech_002364 --> ing13_NiSO4_x_6_H2O
    CHEBI_53001["CHEBI:53001"]
    ing13_NiSO4_x_6_H2O -.-> CHEBI_53001
    ing14_Na2SeO4["Na2SeO4"]
    CultureMech_002364 --> ing14_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing14_Na2SeO4 -.-> CHEBI_77775
    ing15_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002364 --> ing15_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing15_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing16_NaVO3["NaVO3"]
    CultureMech_002364 --> ing16_NaVO3
    CHEBI_75221["CHEBI:75221"]
    ing16_NaVO3 -.-> CHEBI_75221
    ing17_H2SO4["H2SO4"]
    CultureMech_002364 --> ing17_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing17_H2SO4 -.-> CHEBI_26836

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
27.7778 G_PER_L CHEBI:26710
sodium chloride
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.444444 G_PER_L CHEBI:62946
ammonium sulfate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.444444 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 K2HPO4
HO4P.2K 174.174 g/mol
0.444444 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
5 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
137.624 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
6 K2S4O6
2K.O6S4 302.458 g/mol
14.9505 G_PER_L CHEBI:86466
potassium tetrathionate
7 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
10 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
8 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
1 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
9 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
1 G_PER_L CHEBI:86360
manganese(II) sulfate
10 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
11 Cr2(SO4)3 x n H2O
2Cr.3O4S 392.181 g/mol
0.5 G_PER_L CHEBI:53471
chromium(III) sulfate
12 H3BO3
H3BO3 61.833 g/mol
0.6 G_PER_L CHEBI:33118
boric acid
13 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.5 G_PER_L CHEBI:75213
sodium molybdate dihydrate
14 NiSO4 x 6 H2O
Ni.O4S 154.756 g/mol
1 G_PER_L CHEBI:53001
nickel sulfate
15 Na2SeO4
2Na.O4Se 188.936 g/mol
1 G_PER_L CHEBI:77775
sodium selenate
16 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
1 G_PER_L CHEBI:63939
sodium tungstate dihydrate
17 NaVO3
Na.O3V 121.931 g/mol
0.1 G_PER_L CHEBI:75221
sodium metavanadate
18 H2SO4
H2O4S 98.079 g/mol
1000 G_PER_L CHEBI:26836
sulfuric acid

Preparation

  1. Adjust pH 2.5 with H2SO4 and autoclave.
  2. Adjust pH to 2.5 and filter-sterilize. Prepare the solution just prior to use.
  3. Aseptically combine solution A and solution B (freshly prepared). Then, add the following solutions (autoclaved):

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:18.978342Z mediadive-import Imported from MediaDive Source: JCM, ID: J1197
2026-01-28T19:48:13.477107Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:12.727535Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002364
2026-03-17T06:29:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.754317+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ACIDIHALOBACTER PROSPERUS F5 MEDIUM" to "acidihalobacter_prosperus_f5_medium" for consistent matching
2026-06-05T07:40:47.242878+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.470993+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:52.572483+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:21.859326+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 0fa15dd8cd1df654775e68ccd3febf47a4c3877a3318bd1bf9ffc843f1afe4fc