saltwater_medium_for_oceanic_aoa

Overview

External ontology

mediadive.medium:J1198 (source link unavailable) — SALTWATER MEDIUM FOR OCEANIC AOA

Applications

• Microbial cultivation

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1198

Composition

flowchart LR
    CultureMech_002365["saltwater_medium_for_oceanic_aoa"]
    ing0_NaCl["NaCl"]
    CultureMech_002365 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002365 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002365 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002365 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_KBr["KBr"]
    CultureMech_002365 --> ing4_KBr
    CHEBI_32030["CHEBI:32030"]
    ing4_KBr -.-> CHEBI_32030
    ing5_KH2PO4["KH2PO4"]
    CultureMech_002365 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_NaHCO3["NaHCO3"]
    CultureMech_002365 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_FeNa_EDTA["FeNa-EDTA"]
    CultureMech_002365 --> ing7_FeNa_EDTA
    ing8_NH4Cl["NH4Cl"]
    CultureMech_002365 --> ing8_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing8_NH4Cl -.-> CHEBI_31206
    ing9_NaOH["NaOH"]
    CultureMech_002365 --> ing9_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing9_NaOH -.-> CHEBI_32145
    ing10_HEPES["HEPES"]
    CultureMech_002365 --> ing10_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing10_HEPES -.-> CHEBI_46756
    ing11_HCl["HCl"]
    CultureMech_002365 --> ing11_HCl
    CHEBI_17883["CHEBI:17883"]
    ing11_HCl -.-> CHEBI_17883
    ing12_H3BO3["H3BO3"]
    CultureMech_002365 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002365 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002365 --> ing14_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002365 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002365 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002365 --> ing17_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002365 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (19)

Preparation

1. Mix components thoroughly and autoclave. After cooling, aseptically add the following solutions (autoclaved or *filter-sterilized) in the given order:
2. Check pH of the medium to be 7.2. Prior to use, add per liter 1.0 ml catalase solution (5-10 units/ml, filter-sterilized, freshly prepared) to the medium.
3. Use glass or polystyrene containers for cultivation. Cultivate in the dark without shaking.
4. Dissolve NaOH in 300 ml distilled water. While stirring vigorously, slowly add HEPES until completely dissolved. Fill up to 450 ml with distilled water. If 1 ml of this solution is added to 100ml of the medium (containing all additive but HEPES), pH should be around 7.6 at 30C. Otherwise, adjust pH of the HEPES solution with 10 N NaOH or conc. HCl. Finally fill up to 500 ml with distilled water.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• saltwater_medium_for_oceanic_aoa (CultureMech:002365)