natronoarchaea_medium_with_glucosamine

CultureMech:002376 bacterial COMPLEX LIQUID
Original name: NATRONOARCHAEA MEDIUM WITH GLUCOSAMINE

Overview

External ontology
mediadive.medium:J1207 (source link unavailable) — NATRONOARCHAEA MEDIUM WITH GLUCOSAMINE
Applications
  • Microbial cultivation
pH
7.0
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1207

Composition

flowchart LR
    CultureMech_002376["natronoarchaea_medium_with_glucosamine"]
    ing0_MgSO4["MgSO4"]
    CultureMech_002376 --> ing0_MgSO4
    CHEBI_32599["CHEBI:32599"]
    ing0_MgSO4 -.-> CHEBI_32599
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002376 --> ing1_Yeast_extract
    ing2_N_Acetyl_D_glucosamine["N-Acetyl-D-glucosamine"]
    CultureMech_002376 --> ing2_N_Acetyl_D_glucosamine
    CHEBI_506227["CHEBI:506227"]
    ing2_N_Acetyl_D_glucosamine -.-> CHEBI_506227
    ing3_NaCl["NaCl"]
    CultureMech_002376 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_KCl["KCl"]
    CultureMech_002376 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_K2HPO4["K2HPO4"]
    CultureMech_002376 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_NH4Cl["NH4Cl"]
    CultureMech_002376 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_Na2CO3["Na2CO3"]
    CultureMech_002376 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_NaHCO3["NaHCO3"]
    CultureMech_002376 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_Biotin["Biotin"]
    CultureMech_002376 --> ing9_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing9_Biotin -.-> CHEBI_15956
    ing10_Folic_acid["Folic acid"]
    CultureMech_002376 --> ing10_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing10_Folic_acid -.-> CHEBI_27470
    ing11_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002376 --> ing11_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing11_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing12_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002376 --> ing12_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing12_Thiamine_HCl -.-> CHEBI_49105
    ing13_Riboflavin["Riboflavin"]
    CultureMech_002376 --> ing13_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing13_Riboflavin -.-> CHEBI_17015
    ing14_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002376 --> ing14_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing14_Nicotinic_acid -.-> CHEBI_15940
    ing15_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002376 --> ing15_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing15_Calcium_pantothenate -.-> CHEBI_31345
    ing16_Vitamin_B12["Vitamin B12"]
    CultureMech_002376 --> ing16_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing16_Vitamin_B12 -.-> CHEBI_176843
    ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002376 --> ing17_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing18_Lipoic_acid["Lipoic acid"]
    CultureMech_002376 --> ing18_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing18_Lipoic_acid -.-> CHEBI_16494
    ing19_EDTA["EDTA"]
    CultureMech_002376 --> ing19_EDTA
    CHEBI_4735["CHEBI:4735"]
    ing19_EDTA -.-> CHEBI_4735
    ing20_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002376 --> ing20_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing20_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing21_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002376 --> ing21_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing21_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing22_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002376 --> ing22_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing22_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing23_H3BO3["H3BO3"]
    CultureMech_002376 --> ing23_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing23_H3BO3 -.-> CHEBI_33118
    ing24_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002376 --> ing24_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing24_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing25_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002376 --> ing25_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing25_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing26_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002376 --> ing26_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing26_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing27_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002376 --> ing27_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing27_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 MgSO4
Mg.O4S 120.368 g/mol
1 G_PER_L CHEBI:32599
magnesium sulfate
2 Yeast extract 0.2 G_PER_L
3 N-Acetyl-D-glucosamine
C8H15NO6 221.209 g/mol
10 G_PER_L CHEBI:506227
N-acetyl-D-glucosamine
4 NaCl
Cl.Na 58.443 g/mol
256.0 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 240.0, 16.0]
5 KCl
Cl.K 74.551 g/mol
5 G_PER_L CHEBI:32588
potassium chloride
6 K2HPO4
HO4P.2K 174.174 g/mol
3.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 [Merged 2 duplicates: 2.5, 1.0]
7 NH4Cl
Cl.H4N 53.492 g/mol
0.5 G_PER_L CHEBI:31206
ammonium chloride
8 Na2CO3
CO3.2Na 105.988 g/mol
190 G_PER_L CHEBI:29377
sodium carbonate
9 NaHCO3
CHO3.Na 84.006 g/mol
30 G_PER_L CHEBI:32139
sodium hydrogencarbonate
10 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
11 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
12 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
13 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
14 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
15 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
16 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
17 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
18 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
19 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid
20 EDTA
C10H16N2O8 292.244 g/mol
5 G_PER_L CHEBI:4735
ethylenediaminetetraacetic acid
21 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
22 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
23 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
24 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
25 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
26 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
27 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
28 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix two media autoclaved separately. Aseptically add the following solutions (autoclaved or *filter-sterilized):
  2. Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 with 0.5 M K2HPO4 and autoclave.
  3. Add components to distilled water and bring volume to 1.0 L. Autoclave.
  4. Adjust pH to 3.6.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:19.017328Z mediadive-import Imported from MediaDive Source: JCM, ID: J1207
2026-03-13T03:09:04.909576Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:12.829036Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002376
2026-03-17T06:30:10.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:25.870379+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "NATRONOARCHAEA MEDIUM WITH GLUCOSAMINE" to "natronoarchaea_medium_with_glucosamine" for consistent matching
2026-06-05T07:41:39.334947+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.430071+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:51.108475+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 1006c81c33a102bdd1727444aec6f72d4bf4226c16554264943a139968c0a384