Overview
- External ontology
- mediadive.medium:J1219 (source link unavailable) — METHYLOTROPH MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1219
Composition
flowchart LR
CultureMech_002387["methylotroph_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002387 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_NaNO3["NaNO3"]
CultureMech_002387 --> ing1_NaNO3
CHEBI_63005["CHEBI:63005"]
ing1_NaNO3 -.-> CHEBI_63005
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002387 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002387 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_Na2SO4["Na2SO4"]
CultureMech_002387 --> ing4_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing4_Na2SO4 -.-> CHEBI_32149
ing5_HEPES["HEPES"]
CultureMech_002387 --> ing5_HEPES
CHEBI_46756["CHEBI:46756"]
ing5_HEPES -.-> CHEBI_46756
ing6_Methanol["Methanol"]
CultureMech_002387 --> ing6_Methanol
CHEBI_17790["CHEBI:17790"]
ing6_Methanol -.-> CHEBI_17790
ing7_CuCl2["CuCl2"]
CultureMech_002387 --> ing7_CuCl2
CHEBI_49553["CHEBI:49553"]
ing7_CuCl2 -.-> CHEBI_49553
ing8_CeCl3["CeCl3"]
CultureMech_002387 --> ing8_CeCl3
CHEBI_35458["CHEBI:35458"]
ing8_CeCl3 -.-> CHEBI_35458
ing9_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002387 --> ing9_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing9_Nitrilotriacetic_acid -.-> CHEBI_44557
ing10_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_002387 --> ing10_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing10_FeCl3_x_6_H2O -.-> CHEBI_86254
ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002387 --> ing11_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002387 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
ing13_ZnCl2["ZnCl2"]
CultureMech_002387 --> ing13_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing13_ZnCl2 -.-> CHEBI_49976
ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002387 --> ing14_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
ing15_H3BO3["H3BO3"]
CultureMech_002387 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002387 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002387 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002387 --> ing18_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing18_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing19_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002387 --> ing19_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing19_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing20_Biotin["Biotin"]
CultureMech_002387 --> ing20_Biotin
CHEBI_15956["CHEBI:15956"]
ing20_Biotin -.-> CHEBI_15956
ing21_Folic_acid["Folic acid"]
CultureMech_002387 --> ing21_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing21_Folic_acid -.-> CHEBI_27470
ing22_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002387 --> ing22_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing22_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing23_Thiamine_HCl["Thiamine HCl"]
CultureMech_002387 --> ing23_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing23_Thiamine_HCl -.-> CHEBI_49105
ing24_Riboflavin["Riboflavin"]
CultureMech_002387 --> ing24_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing24_Riboflavin -.-> CHEBI_17015
ing25_Nicotinic_acid["Nicotinic acid"]
CultureMech_002387 --> ing25_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing25_Nicotinic_acid -.-> CHEBI_15940
ing26_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002387 --> ing26_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing26_Calcium_pantothenate -.-> CHEBI_31345
ing27_Vitamin_B12["Vitamin B12"]
CultureMech_002387 --> ing27_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing27_Vitamin_B12 -.-> CHEBI_176843
ing28_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002387 --> ing28_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing28_p_Aminobenzoic_acid -.-> CHEBI_30753
ing29_Lipoic_acid["Lipoic acid"]
CultureMech_002387 --> ing29_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing29_Lipoic_acid -.-> CHEBI_16494
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.268657 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
NaNO3
NO3.Na 84.994 g/mol
|
0.41791 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.199005 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.16965170000000002 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.0696517, 0.1] |
| 5 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.0139303 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 6 | HEPES | 4.74627 G_PER_L |
CHEBI:46756
HEPES
|
|
| 7 |
Methanol
CH4O 32.042 g/mol
|
0.81 G_PER_L |
CHEBI:17790
methanol
|
|
| 8 |
CuCl2
Cl2Cu 134.452 g/mol
|
2 G_PER_L |
CHEBI:49553
copper(II) chloride
|
|
| 9 |
CeCl3
CeCl3 246.475 g/mol
|
2 G_PER_L |
CHEBI:35458
cerium trichloride
|
|
| 10 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
12.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 11 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
1.35 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 12 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.024 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 14 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 15 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.025 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.024 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.12 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.004 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 20 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 21 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 22 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 23 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 24 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 25 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 26 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 27 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 28 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 29 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 30 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components and adjust pH to 7.0 with 6 N KOH. For preparation of solid medium, add 15.0 g agar. Autoclave. After cooling, add the following solutions (filer-sterilized):
- Dissolve nitrilotriacetic acid first in 200 ml of distilled water and adjust pH to 6.5 with KOH. Then add the remaining salts and bring volume to 1.0 L. Readjust pH to 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- methylotroph_medium (CultureMech:002387)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.065451Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1219 |
| 2026-03-13T03:08:41.300828Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:12.922547Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002387 |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.972485+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHYLOTROPH MEDIUM" to "methylotroph_medium" for consistent matching |
| 2026-06-05T07:41:33.476738+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.691339+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:45.192819+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 787fcb8b498ea32c10323fb58f8ad86f56011c8928028ec5ccb933a53d37468f |