Overview
- External ontology
- mediadive.medium:J121 (source link unavailable) — BENZOATE MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=121
Composition
flowchart LR
CultureMech_002388["benzoate_medium"]
ing0__NH4_2HPO4["(NH4)2HPO4"]
CultureMech_002388 --> ing0__NH4_2HPO4
CHEBI_63051["CHEBI:63051"]
ing0__NH4_2HPO4 -.-> CHEBI_63051
ing1_KH2PO4["KH2PO4"]
CultureMech_002388 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NaCl["NaCl"]
CultureMech_002388 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002388 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Yeast_extract["Yeast extract"]
CultureMech_002388 --> ing4_Yeast_extract
ing5_Sodium_benzoate["Sodium benzoate"]
CultureMech_002388 --> ing5_Sodium_benzoate
CHEBI_113455["CHEBI:113455"]
ing5_Sodium_benzoate -.-> CHEBI_113455
ing6_Agar["Agar"]
CultureMech_002388 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2HPO4
2H4N.HO4P 132.056 g/mol
|
3 G_PER_L |
CHEBI:63051
diammonium hydrogen phosphate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 | Yeast extract | 0.5 G_PER_L | ||
| 6 |
Sodium benzoate
C7H5O2.Na 144.105 g/mol
|
3 G_PER_L |
CHEBI:113455
sodium benzoate
|
|
| 7 | Agar | 20 G_PER_L |
CHEBI:2509
agar
|
( Noble) |
Preparation
- Separately sterilize a solution of sodium benzoate by filtration, and aseptically add it to the medium.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- benzoate_medium (CultureMech:002388)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.763206Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J121 |
| 2026-03-13T06:19:12.929683Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002388 |
| 2026-03-17T06:30:14.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:25.981107+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BENZOATE MEDIUM" to "benzoate_medium" for consistent matching |
| 2026-06-05T07:40:50.769285+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.587495+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:53.862626+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:24.986425+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint fda674fbebd08f4a9d2c3db7eaa8fe0e1ebf7da26bddb15ae0b7122e6c03fc10 |