zestosphaera_medium

CultureMech:002394 archaea COMPLEX LIQUID
Original name: ZESTOSPHAERA MEDIUM

Overview

External ontology
mediadive.medium:J1227 (source link unavailable) — ZESTOSPHAERA MEDIUM
Applications
  • Microbial cultivation
pH
6.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1227

Composition

flowchart LR
    CultureMech_002394["zestosphaera_medium"]
    ing0_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_002394 --> ing0_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing0_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing1_NaOH["NaOH"]
    CultureMech_002394 --> ing1_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing1_NaOH -.-> CHEBI_32145
    ing2_Resazurin["Resazurin"]
    CultureMech_002394 --> ing2_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing2_Resazurin -.-> CHEBI_8806
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_002394 --> ing3_Yeast_extract
    ing4_Tryptone["Tryptone"]
    CultureMech_002394 --> ing4_Tryptone
    ing5_Casamino_acids["Casamino acids"]
    CultureMech_002394 --> ing5_Casamino_acids
    ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002394 --> ing6_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing7__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002394 --> ing7__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing7__NH4_2SO4 -.-> CHEBI_62946
    ing8_KH2PO4["KH2PO4"]
    CultureMech_002394 --> ing8_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing8_KH2PO4 -.-> CHEBI_63036
    ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002394 --> ing9_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002394 --> ing10_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing11_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_002394 --> ing11_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing11_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002394 --> ing12_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing13_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_002394 --> ing13_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing13_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing14_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002394 --> ing14_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing14_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002394 --> ing15_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002394 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_VOSO4_x_n_H2O["VOSO4 x n H2O"]
    CultureMech_002394 --> ing17_VOSO4_x_n_H2O
    CHEBI_87020["CHEBI:87020"]
    ing17_VOSO4_x_n_H2O -.-> CHEBI_87020
    ing18_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002394 --> ing18_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing18_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing19_Na2_EDTA["Na2-EDTA"]
    CultureMech_002394 --> ing19_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing19_Na2_EDTA -.-> CHEBI_64734
    ing20_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002394 --> ing20_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing20_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing21_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002394 --> ing21_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing21_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing22_ZnCl2["ZnCl2"]
    CultureMech_002394 --> ing22_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing22_ZnCl2 -.-> CHEBI_49976
    ing23_AlCl3_x_6_H2O["AlCl3 x 6 H2O"]
    CultureMech_002394 --> ing23_AlCl3_x_6_H2O
    CHEBI_30115["CHEBI:30115"]
    ing23_AlCl3_x_6_H2O -.-> CHEBI_30115
    ing24_Na2WO2_x_2_H2O["Na2WO2 x 2 H2O"]
    CultureMech_002394 --> ing24_Na2WO2_x_2_H2O
    ing25_NiSO4_x_6_H2O["NiSO4 x 6 H2O"]
    CultureMech_002394 --> ing25_NiSO4_x_6_H2O
    CHEBI_53001["CHEBI:53001"]
    ing25_NiSO4_x_6_H2O -.-> CHEBI_53001
    ing26_H2SeO3["H2SeO3"]
    CultureMech_002394 --> ing26_H2SeO3
    CHEBI_26642["CHEBI:26642"]
    ing26_H2SeO3 -.-> CHEBI_26642
    ing27_H3BO3["H3BO3"]
    CultureMech_002394 --> ing27_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing27_H3BO3 -.-> CHEBI_33118

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
0.995025 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
2 NaOH
HO.Na 39.997 g/mol
0.079602 G_PER_L CHEBI:32145
sodium hydroxide
3 Resazurin
C12H7NO4 229.191 g/mol
0.000497512 G_PER_L CHEBI:8806
Resazurin
4 Yeast extract 0.497512 G_PER_L
5 Tryptone 0.497512 G_PER_L
6 Casamino acids 0.248756 G_PER_L
7 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.0310945 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
8 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.3 G_PER_L CHEBI:62946
ammonium sulfate
9 KH2PO4
H2O4P.K 136.084 g/mol
0.28 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
10 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
11 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.07 G_PER_L CHEBI:86158
calcium chloride dihydrate
12 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.002 G_PER_L CHEBI:86254
iron trichloride hexahydrate
13 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1018 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 [Merged 2 duplicates: 0.0018, 0.1]
14 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.0045 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
15 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.00022 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
16 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.020050000000000002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
 [Merged 2 duplicates: 5e-05, 0.02]
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01003 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 [Merged 2 duplicates: 3e-05, 0.01]
18 VOSO4 x n H2O
(H2O)n.O5SV 181.021 g/mol
3e-05 G_PER_L CHEBI:87020
vanadyl sulfate hydrate
19 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
1e-05 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
20 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.5 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
21 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.15 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
22 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
23 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
24 AlCl3 x 6 H2O
AlCl3.6H2O 241.431 g/mol
0.04 G_PER_L CHEBI:30115
aluminium trichloride hexahydrate
25 Na2WO2 x 2 H2O 0.03 G_PER_L
26 NiSO4 x 6 H2O
Ni.O4S 154.756 g/mol
0.02 G_PER_L CHEBI:53001
nickel sulfate
27 H2SeO3
H2O3Se 128.973 g/mol
0.01 G_PER_L CHEBI:26642
selenous acid
28 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid

Preparation

  1. Heat the medium to boiling while bubbling with a N2-CO2 (4:1, v/v) gas mixture. After boiling, cool the medium in a water bath. Continue bubbling with the same gas mixture. While the medium is still warm, add the following:
  2. After the medium is cooled to room temperature, adjust pH to 6.0 - 6.1. Dispense the medium into culture vessels (e.g., 10 ml in Balch type tubes) under the same gas mixture, seal with butyl rubber stoppers and autoclave.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:19.091230Z mediadive-import Imported from MediaDive Source: JCM, ID: J1227
2026-01-28T19:48:38.490366Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:36.370720Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:12.976368Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002394
2026-03-17T06:30:47.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.032334+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ZESTOSPHAERA MEDIUM" to "zestosphaera_medium" for consistent matching
2026-06-05T07:40:16.641670+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:27.265685+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:46:42.767659+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:20.521989+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint a39095188dba2e729da6273082786f81285e3de983b581d0bf48fa5f18a931b3