Overview
- External ontology
- mediadive.medium:J1230 (source link unavailable) — NSY MEDIUM FOR POLYNUCLEOBACTER
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1230
Composition
flowchart LR
CultureMech_002397["nsy_medium_for_polynucleobacter"]
ing0_Nutrient_broth["Nutrient broth"]
CultureMech_002397 --> ing0_Nutrient_broth
ing1_Phytone_peptone["Phytone peptone"]
CultureMech_002397 --> ing1_Phytone_peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_002397 --> ing2_Yeast_extract
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002397 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
CultureMech_002397 --> ing4_Ca_NO3_2_x_4_H2O
CHEBI_86159["CHEBI:86159"]
ing4_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
ing5_NaHCO3["NaHCO3"]
CultureMech_002397 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_KCl["KCl"]
CultureMech_002397 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_K2HPO4["K2HPO4"]
CultureMech_002397 --> ing7_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing7_K2HPO4 -.-> CHEBI_131527
ing8_Agar["Agar"]
CultureMech_002397 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
ing9_EDTA["EDTA"]
CultureMech_002397 --> ing9_EDTA
CHEBI_4735["CHEBI:4735"]
ing9_EDTA -.-> CHEBI_4735
ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002397 --> ing10_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002397 --> ing11_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002397 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_H3BO3["H3BO3"]
CultureMech_002397 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002397 --> ing14_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002397 --> ing15_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002397 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002397 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Nutrient broth | 1 G_PER_L | ||
| 2 | Phytone peptone | 1 G_PER_L | ||
| 3 | Yeast extract | 1 G_PER_L | ||
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.075 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
|
0.043 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.016 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.005 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 8 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.0037 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 9 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 10 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 11 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 12 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.2 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
[Merged 2 duplicates: 0.1, 0.1] |
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.06 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
[Merged 2 duplicates: 0.03, 0.03] |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.6 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.3, 0.3] |
| 15 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.4 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
[Merged 2 duplicates: 0.2, 0.2] |
| 16 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.02 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
[Merged 2 duplicates: 0.01, 0.01] |
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.04 G_PER_L |
CHEBI:34887
nickel dichloride
|
[Merged 2 duplicates: 0.02, 0.02] |
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.06 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
[Merged 2 duplicates: 0.03, 0.03] |
Preparation
- Adjust pH to 7.2.
- Adjust pH to 3.6.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J1230_NSY_MEDIUM_FOR_POLYNUCLEOBACTER (CultureMech:002397)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.104688Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1230 |
| 2026-03-13T03:09:04.617078Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 7 duplicate ingredient(s) |
| 2026-03-13T06:19:13.000443Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002397 |
| 2026-03-17T06:30:10.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.059203+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NSY MEDIUM FOR POLYNUCLEOBACTER" to "nsy_medium_for_polynucleobacter" for consistent matching |
| 2026-06-05T07:40:16.654858+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.773944+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:35.319333+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 90e2f182ee15e9612ad77f0fdc41bc9a12747abbf45d25c1d063b9ddaba41af6 |