Overview
- External ontology
- mediadive.medium:J1239 (source link unavailable) — THERMOPLASMA KAMCHATKENSIS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 2.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1239
Composition
flowchart LR
CultureMech_002405["thermoplasma_kamchatkensis_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002405 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002405 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002405 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_002405 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_Yeast_extract["Yeast extract"]
CultureMech_002405 --> ing4_Yeast_extract
ing5_Sucrose["Sucrose"]
CultureMech_002405 --> ing5_Sucrose
CHEBI_17992["CHEBI:17992"]
ing5_Sucrose -.-> CHEBI_17992
ing6_Sulfur["Sulfur"]
CultureMech_002405 --> ing6_Sulfur
CHEBI_26833["CHEBI:26833"]
ing6_Sulfur -.-> CHEBI_26833
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 5 | Yeast extract | 0.1 G_PER_L | ||
| 6 |
Sucrose
C12H22O11 342.297 g/mol
|
5 G_PER_L |
CHEBI:17992
sucrose
|
|
| 7 |
Sulfur
S 32.067 g/mol
|
4 G_PER_L |
CHEBI:26833
sulfur atom
|
Preparation
- Mix components except sulfur, yeast extract and sucrose, adjust pH to 2.5 with 10 N H2SO4 and autoclave. Separately autoclave yeast extract and sucrose and add to the medium. Steam sulfur for 3 hr on each of 3 successive days. Aseptically distribute the medium into culture vessels (e.g., 10 ml in Balch tubes) containing appropriate amount of sulfur, replace gas phase with a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- thermoplasma_kamchatkensis_medium (CultureMech:002405)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.132861Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1239 |
| 2026-03-13T06:19:13.041342Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002405 |
| 2026-03-17T06:30:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.105006+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "THERMOPLASMA KAMCHATKENSIS MEDIUM" to "thermoplasma_kamchatkensis_medium" for consistent matching |
| 2026-06-05T07:41:50.410233+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:13.113554+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:32.964567+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 57d65c1c2c6548373c64bf387713ae8ebfdc5685e3aa7325f00c1cd135ad895b |