Overview
- External ontology
- mediadive.medium:J1242 (source link unavailable) — ANAEROBACULUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1242
Composition
flowchart LR
CultureMech_002409["anaerobaculum_medium"]
ing0_Trypticase_peptone["Trypticase peptone"]
CultureMech_002409 --> ing0_Trypticase_peptone
ing1_Peptone["Peptone"]
CultureMech_002409 --> ing1_Peptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_002409 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_002409 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_Resazurin["Resazurin"]
CultureMech_002409 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002409 --> ing5_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing5_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing6_Glucose["Glucose"]
CultureMech_002409 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
ing7_Na2S2O3["Na2S2O3"]
CultureMech_002409 --> ing7_Na2S2O3
CHEBI_132112["CHEBI:132112"]
ing7_Na2S2O3 -.-> CHEBI_132112
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002409 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002409 --> ing9_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
ing10_K2HPO4["K2HPO4"]
CultureMech_002409 --> ing10_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing10_K2HPO4 -.-> CHEBI_131527
ing11_KH2PO4["KH2PO4"]
CultureMech_002409 --> ing11_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing11_KH2PO4 -.-> CHEBI_63036
ing12_NaHCO3["NaHCO3"]
CultureMech_002409 --> ing12_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing12_NaHCO3 -.-> CHEBI_32139
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase peptone | 4.87329 G_PER_L | ||
| 2 | Peptone | 4.87329 G_PER_L | ||
| 3 | Yeast extract | 9.74659 G_PER_L | ||
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
9.797270000000001 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 7.79727, 2.0] |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000487329 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
10 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 7 |
Glucose
C6H12O6 180.156 g/mol
|
6 G_PER_L |
CHEBI:17234
glucose
|
|
| 8 |
Na2S2O3
2Na.O3S2 158.111 g/mol
|
10 G_PER_L |
CHEBI:132112
sodium thiosulfate
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 11 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 12 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 13 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Preparation
- Mix components thoroughly, bring to a boil and cool down under a stream of N2. While gassing, add the following solutions and adjust pH to 7.0:
- Distribute the medium into culture vessels (e.g., 10 ml in Balch type tubes) under N2, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions (autoclaved and stored under a N2 atmosphere):
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J1242_ANAEROBACULUM_MEDIUM (CultureMech:002409)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.139846Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1242 |
| 2026-03-13T03:09:16.231698Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:13.060179Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002409 |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.126021+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ANAEROBACULUM MEDIUM" to "anaerobaculum_medium" for consistent matching |
| 2026-06-05T07:40:16.664063+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.067926+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:42.778389+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:35.347839+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint cc875d30f7f737ae4e4c31493a2db7d02e5bf69e98dcf231f9cbaf00123df1b4 |