Overview
- External ontology
- mediadive.medium:J1252 (source link unavailable) — HALOARCHAEAL COMPLEX MEDIUM PH 7.0
- Applications
-
- Microbial cultivation
- pH
- 7.0
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1252
Composition
flowchart LR
CultureMech_002418["haloarchaeal_complex_medium_ph_7_0"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002418 --> ing0_Yeast_extract
ing1_Fish_peptone["Fish peptone"]
CultureMech_002418 --> ing1_Fish_peptone
ing2_Sodium_formate["Sodium formate"]
CultureMech_002418 --> ing2_Sodium_formate
CHEBI_62965["CHEBI:62965"]
ing2_Sodium_formate -.-> CHEBI_62965
ing3_Sodium_acetate["Sodium acetate"]
CultureMech_002418 --> ing3_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing3_Sodium_acetate -.-> CHEBI_32954
ing4_Sodium_lactate["Sodium lactate"]
CultureMech_002418 --> ing4_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing4_Sodium_lactate -.-> CHEBI_75228
ing5_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002418 --> ing5_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing5_Sodium_pyruvate -.-> CHEBI_50144
ing6_Sodium_succinate["Sodium succinate"]
CultureMech_002418 --> ing6_Sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing6_Sodium_succinate -.-> CHEBI_63675
ing7_Sodium_malate["Sodium malate"]
CultureMech_002418 --> ing7_Sodium_malate
CHEBI_91260["CHEBI:91260"]
ing7_Sodium_malate -.-> CHEBI_91260
ing8_Sodium_fumarate["Sodium fumarate"]
CultureMech_002418 --> ing8_Sodium_fumarate
CHEBI_115156["CHEBI:115156"]
ing8_Sodium_fumarate -.-> CHEBI_115156
ing9_Sodium_citrate["Sodium citrate"]
CultureMech_002418 --> ing9_Sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing9_Sodium_citrate -.-> CHEBI_53258
ing10_KCl["KCl"]
CultureMech_002418 --> ing10_KCl
CHEBI_32588["CHEBI:32588"]
ing10_KCl -.-> CHEBI_32588
ing11_K2HPO4["K2HPO4"]
CultureMech_002418 --> ing11_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing11_K2HPO4 -.-> CHEBI_131527
ing12_CaCl2_x_H2O["CaCl2 x H2O"]
CultureMech_002418 --> ing12_CaCl2_x_H2O
CHEBI_3312["CHEBI:3312"]
ing12_CaCl2_x_H2O -.-> CHEBI_3312
ing13_NH4Cl["NH4Cl"]
CultureMech_002418 --> ing13_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing13_NH4Cl -.-> CHEBI_31206
ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002418 --> ing14_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
ing15_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002418 --> ing15_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing15_MgCl2_x_6_H2O -.-> CHEBI_86345
ing16_NaCl["NaCl"]
CultureMech_002418 --> ing16_NaCl
CHEBI_26710["CHEBI:26710"]
ing16_NaCl -.-> CHEBI_26710
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.05 G_PER_L | ||
| 2 | Fish peptone | 0.25 G_PER_L | ||
| 3 |
Sodium formate
CHO2.Na 68.007 g/mol
|
0.2 G_PER_L |
CHEBI:62965
sodium formate
|
|
| 4 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.2 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 5 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
0.2 G_PER_L |
CHEBI:75228
sodium lactate
|
|
| 6 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.2 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 7 |
Sodium succinate
C4H4O4.2Na 162.052 g/mol
|
0.2 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
|
| 8 | Sodium malate | 0.2 G_PER_L |
CHEBI:91260
disodium malate
|
|
| 9 |
Sodium fumarate
C4H2O4.2Na 160.036 g/mol
|
0.2 G_PER_L |
CHEBI:115156
disodium fumarate
|
|
| 10 |
Sodium citrate
C6H5O7.3Na 258.069 g/mol
|
0.2 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 11 |
KCl
Cl.K 74.551 g/mol
|
5.4 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 12 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 13 |
CaCl2 x H2O
Ca.2Cl 110.984 g/mol
|
0.29 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 14 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.27 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 15 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
26.8 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 16 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
23 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 17 |
NaCl
Cl.Na 58.443 g/mol
|
184 G_PER_L |
CHEBI:26710
sodium chloride
|
Preparation
- Dissolve components in distilled water and bring volume to 1.0 L. Adjust pH to 7.0. For preparation of solid medium, add 20.0 g/L agar (if necessary, use washed agar as described in the recipe of Medium No. 574).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- haloarchaeal_complex_medium_ph_7_0 (CultureMech:002418)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.178660Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1252 |
| 2026-03-13T06:19:13.148932Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002418 |
| 2026-03-17T06:30:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.225854+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALOARCHAEAL COMPLEX MEDIUM PH 7.0" to "haloarchaeal_complex_medium_ph_7_0" for consistent matching |
| 2026-06-05T07:41:07.452106+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.425078+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:28.633597+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0e7441227a470a460532d48129f7bac59c048d2bced7532a7f142deffdd020dd |