Overview
- External ontology
- mediadive.medium:J1264 (source link unavailable) — JCM MEDIUM No. 1264
- Applications
-
- Microbial cultivation
- pH
- 10.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1264
Composition
flowchart LR
CultureMech_002430["jcm_medium_no_1264"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002430 --> ing0_Yeast_extract
ing1_Sodium_formate["Sodium formate"]
CultureMech_002430 --> ing1_Sodium_formate
CHEBI_62965["CHEBI:62965"]
ing1_Sodium_formate -.-> CHEBI_62965
ing2_Sodium_acetate["Sodium acetate"]
CultureMech_002430 --> ing2_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing2_Sodium_acetate -.-> CHEBI_32954
ing3_Bacto_peptone["Bacto peptone"]
CultureMech_002430 --> ing3_Bacto_peptone
ing4_Sodium_lactate["Sodium lactate"]
CultureMech_002430 --> ing4_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing4_Sodium_lactate -.-> CHEBI_75228
ing5_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002430 --> ing5_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing5_Sodium_pyruvate -.-> CHEBI_50144
ing6_Disodium_succinate_x_6_H2O["Disodium succinate x 6 H2O"]
CultureMech_002430 --> ing6_Disodium_succinate_x_6_H2O
CHEBI_63686["CHEBI:63686"]
ing6_Disodium_succinate_x_6_H2O -.-> CHEBI_63686
ing7_DL_Malic_acid["DL-Malic acid"]
CultureMech_002430 --> ing7_DL_Malic_acid
CHEBI_6650["CHEBI:6650"]
ing7_DL_Malic_acid -.-> CHEBI_6650
ing8_Fumaric_acid["Fumaric acid"]
CultureMech_002430 --> ing8_Fumaric_acid
CHEBI_18012["CHEBI:18012"]
ing8_Fumaric_acid -.-> CHEBI_18012
ing9_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_002430 --> ing9_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing9_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing10_KCl["KCl"]
CultureMech_002430 --> ing10_KCl
CHEBI_32588["CHEBI:32588"]
ing10_KCl -.-> CHEBI_32588
ing11_K2HPO4["K2HPO4"]
CultureMech_002430 --> ing11_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing11_K2HPO4 -.-> CHEBI_131527
ing12_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002430 --> ing12_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing12_CaCl2_x_2_H2O -.-> CHEBI_86158
ing13_NaNO3["NaNO3"]
CultureMech_002430 --> ing13_NaNO3
CHEBI_63005["CHEBI:63005"]
ing13_NaNO3 -.-> CHEBI_63005
ing14_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002430 --> ing14_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing14_MgCl2_x_6_H2O -.-> CHEBI_86345
ing15_Na2CO3["Na2CO3"]
CultureMech_002430 --> ing15_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing15_Na2CO3 -.-> CHEBI_29377
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.05 G_PER_L | ||
| 2 |
Sodium formate
CHO2.Na 68.007 g/mol
|
0.2 G_PER_L |
CHEBI:62965
sodium formate
|
|
| 3 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.2 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 4 | Bacto peptone | 0.25 G_PER_L | ||
| 5 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
0.2 G_PER_L |
CHEBI:75228
sodium lactate
|
|
| 6 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.2 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 7 |
Disodium succinate x 6 H2O
C4H4O4.6H2O.2Na 270.142 g/mol
|
0.2 G_PER_L |
CHEBI:63686
sodium succinate hexahydrate
|
|
| 8 |
DL-Malic acid
C4H6O5 134.087 g/mol
|
0.2 G_PER_L |
CHEBI:6650
malic acid
|
|
| 9 |
Fumaric acid
C4H4O4 116.072 g/mol
|
0.2 G_PER_L |
CHEBI:18012
fumaric acid
|
|
| 10 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
0.2 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 11 |
KCl
Cl.K 74.551 g/mol
|
5.4 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 12 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 13 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.29 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 14 |
NaNO3
NO3.Na 84.994 g/mol
|
0.27 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 15 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 16 |
Na2CO3
CO3.2Na 105.988 g/mol
|
9 G_PER_L |
CHEBI:29377
sodium carbonate
|
Preparation
- After autoclaving, adjust pH to 10.0 with sterilized 10% Na2CO3 solution.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- jcm_medium_no_1264 (CultureMech:002430)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.221080Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1264 |
| 2026-01-28T19:48:29.839890Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:13.250783Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002430 |
| 2026-03-17T06:30:28.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.344617+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JCM MEDIUM No. 1264" to "jcm_medium_no_1264" for consistent matching |
| 2026-06-05T07:41:09.818416+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.187694+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:38.976886+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b247ea577c02c81630b1dd5f1138a0d6add549b0fab9ab12c6abf4e82b080a38 |