Overview
- External ontology
- mediadive.medium:J1273 (source link unavailable) — 0.01% LB SEAWATER MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.0
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1273 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_002439["0_01_lb_seawater_medium"]
ing0_NaNO3["NaNO3"]
CultureMech_002439 --> ing0_NaNO3
CHEBI_63005["CHEBI:63005"]
ing0_NaNO3 -.-> CHEBI_63005
ing1_K2HPO4["K2HPO4"]
CultureMech_002439 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_Vitamin_B12["Vitamin B12"]
CultureMech_002439 --> ing2_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing2_Vitamin_B12 -.-> CHEBI_176843
ing3_Biotin["Biotin"]
CultureMech_002439 --> ing3_Biotin
CHEBI_15956["CHEBI:15956"]
ing3_Biotin -.-> CHEBI_15956
ing4_Thiamine_HCl["Thiamine HCl"]
CultureMech_002439 --> ing4_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing4_Thiamine_HCl -.-> CHEBI_49105
ing5_Fe_III__EDTA["Fe(III)-EDTA"]
CultureMech_002439 --> ing5_Fe_III__EDTA
CHEBI_30729["CHEBI:30729"]
ing5_Fe_III__EDTA -.-> CHEBI_30729
ing6_Mn_II__x_EDTA["Mn(II) x EDTA"]
CultureMech_002439 --> ing6_Mn_II__x_EDTA
ing7_Tris_hydroxymethyl_aminomethane["Tris(hydroxymethyl)aminomethane"]
CultureMech_002439 --> ing7_Tris_hydroxymethyl_aminomethane
CHEBI_9754["CHEBI:9754"]
ing7_Tris_hydroxymethyl_aminomethane -.-> CHEBI_9754
ing8_Tryptone["Tryptone"]
CultureMech_002439 --> ing8_Tryptone
ing9_Yeast_extract["Yeast extract"]
CultureMech_002439 --> ing9_Yeast_extract
ing10_Sodium_chloride["Sodium chloride"]
CultureMech_002439 --> ing10_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing10_Sodium_chloride -.-> CHEBI_26710
ing11_Sea_water["Sea water"]
CultureMech_002439 --> ing11_Sea_water
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaNO3
NO3.Na 84.994 g/mol
|
0.12 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.005 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 | Vitamin B12 | 1 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 4 |
Biotin
C10H16N2O3S 244.316 g/mol
|
1 G_PER_L |
CHEBI:15956
biotin
|
|
| 5 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
100 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 6 |
Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
|
259 G_PER_L |
CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
|
|
| 7 | Mn(II) x EDTA | 332 G_PER_L | ||
| 8 |
Tris(hydroxymethyl)aminomethane
C4H11NO3 121.136 g/mol
|
1 G_PER_L |
CHEBI:9754
tris
|
|
| 9 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 10 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 11 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 12 | Sea water | 1000 G_PER_L |
Preparation
- Mix components thoroughly, adjust pH to 8.0 with HCl and autoclave.
- Comment: Artificial sea water (such as artificial sea water in Medium No. 1118) can be used instead of natural sea water.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- 0_01_lb_seawater_medium (CultureMech:002439)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.251848Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1273 |
| 2026-03-13T06:19:13.321778Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002439 |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.425634+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "0.01% LB SEAWATER MEDIUM" to "0_01_lb_seawater_medium" for consistent matching |
| 2026-06-05T07:40:15.238806+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.430860+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:33.099358+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 73c6b095f2a8acc2cb405b1476b61f3e309977a783a8a41894793ed7d8dd781a |