Overview
- External ontology
- mediadive.medium:J1282 (source link unavailable) — HNN MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1282
Composition
flowchart LR
CultureMech_002449["hnn_medium"]
ing0_NaCl["NaCl"]
CultureMech_002449 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002449 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002449 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_KCl["KCl"]
CultureMech_002449 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NH4Cl["NH4Cl"]
CultureMech_002449 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4["K2HPO4"]
CultureMech_002449 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002449 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_NaHCO3["NaHCO3"]
CultureMech_002449 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002449 --> ing8_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
ing9_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002449 --> ing9_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing9_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing10_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002449 --> ing10_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing10_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002449 --> ing11_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
ing12_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002449 --> ing12_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing12_MnSO4_x_n_H2O -.-> CHEBI_86360
ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002449 --> ing13_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
ing14_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002449 --> ing14_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing14_CoSO4_x_7_H2O -.-> CHEBI_91244
ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002449 --> ing15_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing16_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002449 --> ing16_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing16_CuSO4_x_5_H2O -.-> CHEBI_31440
ing17_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002449 --> ing17_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing17_AlK_SO4_2 -.-> CHEBI_86463
ing18_H3BO3["H3BO3"]
CultureMech_002449 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002449 --> ing19_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (20)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
25.7525 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 24.7525, 1.0] |
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.15842 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.36634 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 3.36634, 3.0] |
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.49505 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.247525 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.138614 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.793069 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.693069, 0.1] |
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.990099 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 10 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 11 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 12 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 13 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 14 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 15 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 16 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 17 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 18 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Mix components, except NaHCO3, and autoclave under N2 atmosphere. Filter-sterilize 8% (w/v) NaHCO3 solution and add to the medium. Aseptically distribute the medium into culture vessels and seal with butyl rubber stoppers. Replace gas phase with N2O, After inoculation, pressurize the inoculated bottles to 300 kPa H2-CO2 (4:1, v/v).
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- hnn_medium (CultureMech:002449)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.281130Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1282 |
| 2026-03-13T03:09:34.710367Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:13.396822Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002449 |
| 2026-03-17T06:30:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.509220+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HNN MEDIUM" to "hnn_medium" for consistent matching |
| 2026-06-05T07:41:08.659425+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:59.797271+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:37.742217+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 6f840a26929e01f0f08998026f03d8e4cfd0c7d89b8c70bdd6bbb1cdbfa2ae4d |