siuc_3_medium

CultureMech:002450 bacterial COMPLEX LIQUID
Original name: SIUC-3 MEDIUM

Overview

External ontology
mediadive.medium:J1283 (source link unavailable) — SIUC-3 MEDIUM
Applications
  • Microbial cultivation
pH
6.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1283

Composition

flowchart LR
    CultureMech_002450["siuc_3_medium"]
    ing0_HEPES["HEPES"]
    CultureMech_002450 --> ing0_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing0_HEPES -.-> CHEBI_46756
    ing1_Na2SO4["Na2SO4"]
    CultureMech_002450 --> ing1_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing1_Na2SO4 -.-> CHEBI_32149
    ing2_K2HPO4["K2HPO4"]
    CultureMech_002450 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002450 --> ing3_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing4_KCl["KCl"]
    CultureMech_002450 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_NH4Cl["NH4Cl"]
    CultureMech_002450 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_NaHCO3["NaHCO3"]
    CultureMech_002450 --> ing6_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing6_NaHCO3 -.-> CHEBI_32139
    ing7_H3BO3["H3BO3"]
    CultureMech_002450 --> ing7_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing7_H3BO3 -.-> CHEBI_33118
    ing8_Sodium_formate["Sodium formate"]
    CultureMech_002450 --> ing8_Sodium_formate
    CHEBI_62965["CHEBI:62965"]
    ing8_Sodium_formate -.-> CHEBI_62965
    ing9_Yeast_extract["Yeast extract"]
    CultureMech_002450 --> ing9_Yeast_extract
    ing10_Resazurin["Resazurin"]
    CultureMech_002450 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002450 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002450 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_Na2SeO3["Na2SeO3"]
    CultureMech_002450 --> ing13_Na2SeO3
    CHEBI_48843["CHEBI:48843"]
    ing13_Na2SeO3 -.-> CHEBI_48843
    ing14_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002450 --> ing14_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing14_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing15_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002450 --> ing15_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing15_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing16_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002450 --> ing16_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing16_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing17_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002450 --> ing17_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing17_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing18_NaCl["NaCl"]
    CultureMech_002450 --> ing18_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing18_NaCl -.-> CHEBI_26710
    ing19_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002450 --> ing19_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing19_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing20_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002450 --> ing20_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing20_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing21_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002450 --> ing21_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing21_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing22_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002450 --> ing22_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing22_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing23_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002450 --> ing23_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing23_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing24_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002450 --> ing24_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing24_AlK_SO4_2 -.-> CHEBI_86463
    ing25_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002450 --> ing25_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing25_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (26)

# Ingredient Concentration Ontology Notes
1 HEPES 3.56083 G_PER_L CHEBI:46756
HEPES
2 Na2SO4
2Na.O4S 142.043 g/mol
3.20475 G_PER_L CHEBI:32149
sodium sulfate
3 K2HPO4
HO4P.2K 174.174 g/mol
0.16815 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.395648 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
5 KCl
Cl.K 74.551 g/mol
0.49456 G_PER_L CHEBI:32588
potassium chloride
6 NH4Cl
Cl.H4N 53.492 g/mol
0.296736 G_PER_L CHEBI:31206
ammonium chloride
7 NaHCO3
CHO3.Na 84.006 g/mol
0.24728 G_PER_L CHEBI:32139
sodium hydrogencarbonate
8 H3BO3
H3BO3 61.833 g/mol
0.01593472 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.00593472, 0.01]
9 Sodium formate
CHO2.Na 68.007 g/mol
0.672601 G_PER_L CHEBI:62965
sodium formate
10 Yeast extract 0.098912 G_PER_L
11 Resazurin
C12H7NO4 229.191 g/mol
0.000197824 G_PER_L CHEBI:8806
Resazurin
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
14 Na2SeO3
2Na.O3Se 172.937 g/mol
0.001 G_PER_L CHEBI:48843
disodium selenite
15 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.01 G_PER_L CHEBI:63939
sodium tungstate dihydrate
16 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
17 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
18 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
19 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
20 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
21 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
22 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
23 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
24 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
25 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
26 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components thoroughly and adjust pH to 6.5. Distribute the medium into culture vessels (e.g., 25 ml in 120 ml-serum bottles) under a stream of a N2 gas, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solution (autoclaved) :
  2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:19.285056Z mediadive-import Imported from MediaDive Source: JCM, ID: J1283
2026-03-13T03:09:29.873198Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:13.408450Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002450
2026-03-17T06:30:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.521669+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SIUC-3 MEDIUM" to "siuc_3_medium" for consistent matching
2026-06-05T07:41:45.881339+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.501585+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:55.772511+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 61677498b3c565a7308aa4661e9da505b068a3eca428114779c90caa6b78f039