mq116_medium

CultureMech:002454 bacterial COMPLEX LIQUID
Original name: MQ116 MEDIUM

Overview

External ontology
mediadive.medium:J1292 (source link unavailable) — MQ116 MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1292

Composition

flowchart LR
    CultureMech_002454["mq116_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002454 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_KCl["KCl"]
    CultureMech_002454 --> ing1_KCl
    CHEBI_32588["CHEBI:32588"]
    ing1_KCl -.-> CHEBI_32588
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002454 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_KH2PO4["KH2PO4"]
    CultureMech_002454 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_Na2HPO4["Na2HPO4"]
    CultureMech_002454 --> ing4_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing4_Na2HPO4 -.-> CHEBI_34683
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002454 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002454 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_002454 --> ing7_Yeast_extract
    ing8_Beef_extract["Beef extract"]
    CultureMech_002454 --> ing8_Beef_extract
    ing9_Resazurin["Resazurin"]
    CultureMech_002454 --> ing9_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Resazurin -.-> CHEBI_8806
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002454 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_002454 --> ing11_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing11_Sodium_pyruvate -.-> CHEBI_50144
    ing12_Rumen_fluid["Rumen fluid"]
    CultureMech_002454 --> ing12_Rumen_fluid
    ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002454 --> ing13_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing14_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_002454 --> ing14_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing14_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002454 --> ing15_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002454 --> ing16_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing17_ZnCl2["ZnCl2"]
    CultureMech_002454 --> ing17_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing17_ZnCl2 -.-> CHEBI_49976
    ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002454 --> ing18_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing19_H3BO3["H3BO3"]
    CultureMech_002454 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002454 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002454 --> ing21_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing21_NiCl2_x_6_H2O -.-> CHEBI_34887

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
0.29383 G_PER_L CHEBI:26710
sodium chloride
2 KCl
Cl.K 74.551 g/mol
0.0979432 G_PER_L CHEBI:32588
potassium chloride
3 NH4Cl
Cl.H4N 53.492 g/mol
0.29383 G_PER_L CHEBI:31206
ammonium chloride
4 KH2PO4
H2O4P.K 136.084 g/mol
0.29383 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 Na2HPO4
HO4P.2Na 141.958 g/mol
0.29383 G_PER_L CHEBI:34683
disodium hydrogenphosphate
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.195886 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.19794319999999999 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.0979432, 0.1]
8 Yeast extract 4.89716 G_PER_L
9 Beef extract 2.9383 G_PER_L
10 Resazurin
C12H7NO4 229.191 g/mol
0.000489716 G_PER_L CHEBI:8806
Resazurin
11 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.489716 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
12 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
10 G_PER_L CHEBI:50144
sodium pyruvate
13 Rumen fluid 10 G_PER_L ( clarified)
14 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
12.8 G_PER_L CHEBI:44557
nitrilotriacetic acid
15 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
1.35 G_PER_L CHEBI:86254
iron trichloride hexahydrate
16 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
17 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.024 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
18 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
19 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.025 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
20 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.024 G_PER_L CHEBI:75213
sodium molybdate dihydrate
22 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.12 G_PER_L CHEBI:34887
nickel dichloride

Preparation

  1. Mix components thoroughly and adjust pH to 7.0. Distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottle) under a N2 gas atmosphere, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under N2)
  2. Dissolve nitrilotriacetic acid first in 200 ml of distilled water and adjust pH to 6.5 with KOH. Then add the remaining salts and bring volume to 1.0 L. Readjust pH to 7.0 with KOH.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:19.329728Z mediadive-import Imported from MediaDive Source: JCM, ID: J1292
2026-03-13T03:09:06.697668Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:13.451500Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002454
2026-03-17T06:30:13.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.569529+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MQ116 MEDIUM" to "mq116_medium" for consistent matching
2026-06-05T07:41:38.350467+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:09.145049+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:50.327032+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 05c541198594d9330d447511c0c030bd54fbfa489f85cadab0cedc50e3108390