Overview
- External ontology
- mediadive.medium:J1294 (source link unavailable) — MMJ/KT2 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1294
Composition
flowchart LR
CultureMech_002456["mmj_kt2_medium"]
ing0_Sea_water["Sea water"]
CultureMech_002456 --> ing0_Sea_water
ing1_Resazurin["Resazurin"]
CultureMech_002456 --> ing1_Resazurin
CHEBI_8806["CHEBI:8806"]
ing1_Resazurin -.-> CHEBI_8806
ing2_NaHCO3["NaHCO3"]
CultureMech_002456 --> ing2_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing2_NaHCO3 -.-> CHEBI_32139
ing3_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002456 --> ing3_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing3_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing4_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002456 --> ing4_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing4_Na2S_x_9_H2O -.-> CHEBI_76209
ing5_NaCl["NaCl"]
CultureMech_002456 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002456 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002456 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_KCl["KCl"]
CultureMech_002456 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_NH4Cl["NH4Cl"]
CultureMech_002456 --> ing9_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing9_NH4Cl -.-> CHEBI_31206
ing10_K2HPO4["K2HPO4"]
CultureMech_002456 --> ing10_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing10_K2HPO4 -.-> CHEBI_131527
ing11_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002456 --> ing11_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing11_CaCl2_x_2_H2O -.-> CHEBI_86158
ing12_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002456 --> ing12_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing12_FeSO4_x_7_H2O -.-> CHEBI_75836
ing13_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002456 --> ing13_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing13_NiCl2_x_6_H2O -.-> CHEBI_34887
ing14_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002456 --> ing14_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing14_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing15_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002456 --> ing15_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing15_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing16_Biotin["Biotin"]
CultureMech_002456 --> ing16_Biotin
CHEBI_15956["CHEBI:15956"]
ing16_Biotin -.-> CHEBI_15956
ing17_Folic_acid["Folic acid"]
CultureMech_002456 --> ing17_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing17_Folic_acid -.-> CHEBI_27470
ing18_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002456 --> ing18_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing18_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing19_Thiamine_HCl["Thiamine HCl"]
CultureMech_002456 --> ing19_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing19_Thiamine_HCl -.-> CHEBI_49105
ing20_Riboflavin["Riboflavin"]
CultureMech_002456 --> ing20_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing20_Riboflavin -.-> CHEBI_17015
ing21_Nicotinic_acid["Nicotinic acid"]
CultureMech_002456 --> ing21_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing21_Nicotinic_acid -.-> CHEBI_15940
ing22_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002456 --> ing22_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing22_Calcium_pantothenate -.-> CHEBI_31345
ing23_Vitamin_B12["Vitamin B12"]
CultureMech_002456 --> ing23_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing23_Vitamin_B12 -.-> CHEBI_176843
ing24_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002456 --> ing24_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing24_p_Aminobenzoic_acid -.-> CHEBI_30753
ing25_Lipoic_acid["Lipoic acid"]
CultureMech_002456 --> ing25_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing25_Lipoic_acid -.-> CHEBI_16494
ing26_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002456 --> ing26_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing26_Nitrilotriacetic_acid -.-> CHEBI_44557
ing27_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002456 --> ing27_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing27_MnSO4_x_n_H2O -.-> CHEBI_86360
ing28_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002456 --> ing28_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing28_CoSO4_x_7_H2O -.-> CHEBI_91244
ing29_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002456 --> ing29_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing29_ZnSO4_x_7_H2O -.-> CHEBI_32312
CultureMech_002456 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sea water | 1000 G_PER_L | ||
| 2 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000958773 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 3 |
NaHCO3
CHO3.Na 84.006 g/mol
|
12.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 4 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
10 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 5 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
25.7525 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 24.7525, 1.0] |
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.15842 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.36634 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 3.36634, 3.0] |
| 9 |
KCl
Cl.K 74.551 g/mol
|
0.49505 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 10 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.247525 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 11 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.138614 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 12 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.793069 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.693069, 0.1] |
| 13 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.119802 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
[Merged 2 duplicates: 0.019802, 0.1] |
| 14 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.075 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 15 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.05 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 16 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.1 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 17 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 18 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 19 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 20 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 21 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 22 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 23 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 24 | Vitamin B12 | 0.050100000000000006 G_PER_L |
CHEBI:176843
vitamin B12
|
[Merged 2 duplicates: 0.0001, 0.05] |
| 25 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 26 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 27 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 28 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 29 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 30 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 31 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 32 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 33 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 34 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust pH to 7.0 and autoclave. After cooling, add the following solutions (filter-sterilized):
- Aseptically distribute the medium into culture vessels under a stream of H2-CO2 (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solutions (autoclaved and stored under N2):
- Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mmj_kt2_medium (CultureMech:002456)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.337737Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1294 |
| 2026-03-13T03:09:29.624920Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:19:13.476299Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002456 |
| 2026-03-17T06:30:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.596859+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMJ/KT2 MEDIUM" to "mmj_kt2_medium" for consistent matching |
| 2026-06-05T07:41:35.471303+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.277997+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:47.182343+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b3f927c135fbfa7caab162430924290d860b7d315ec1741eff5a4f66b59d84b3 |