Overview
- External ontology
- mediadive.medium:J1295 (source link unavailable) — ALKALINE CLOSTRIDIAL NUTRIENT MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1295
Composition
flowchart LR
CultureMech_002457["alkaline_clostridial_nutrient_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002457 --> ing0_Yeast_extract
ing1_Peptone["Peptone"]
CultureMech_002457 --> ing1_Peptone
ing2_Beef_extract["Beef extract"]
CultureMech_002457 --> ing2_Beef_extract
ing3_Glucose["Glucose"]
CultureMech_002457 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002457 --> ing4_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing4_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing5_NaCl["NaCl"]
CultureMech_002457 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_Sodium_acetate["Sodium acetate"]
CultureMech_002457 --> ing6_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing6_Sodium_acetate -.-> CHEBI_32954
ing7_Starch["Starch"]
CultureMech_002457 --> ing7_Starch
CHEBI_28017["CHEBI:28017"]
ing7_Starch -.-> CHEBI_28017
ing8_Na2CO3["Na2CO3"]
CultureMech_002457 --> ing8_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing8_Na2CO3 -.-> CHEBI_29377
ing9_NaHCO3["NaHCO3"]
CultureMech_002457 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 3 G_PER_L | ||
| 2 | Peptone | 5 G_PER_L | ||
| 3 | Beef extract | 10 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
3 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 8 | Starch | 1 G_PER_L |
CHEBI:28017
starch
|
|
| 9 |
Na2CO3
CO3.2Na 105.988 g/mol
|
7 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.9 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Preparation
- Solution A:
- Adjust pH to 6.5-7.0.
- Solution B:
- Separately autoclave Solutions A and B, and combine after cooling. Cultivate under a static culture condition or a N2 atmosphere. For preparation of solid medium, add 10.0 g gellan gum in Solution A (900 ml). After autoclaving, mix the two solutions while being hot (60 to 70 C) and pour into test tubes or petri dishes.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- alkaline_clostridial_nutrient_medium (CultureMech:002457)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.344308Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1295 |
| 2026-03-13T06:19:13.485216Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002457 |
| 2026-03-17T06:29:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.607106+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ALKALINE CLOSTRIDIAL NUTRIENT MEDIUM" to "alkaline_clostridial_nutrient_medium" for consistent matching |
| 2026-06-05T07:40:47.853405+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.788594+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:22.348594+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1658e98a5de4031975f527973178acab136285ae816eaee7c115a36381bd6e67 |