Overview
- External ontology
- mediadive.medium:J1298 (source link unavailable) — ABSS2
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1298
Composition
flowchart LR
CultureMech_002460["abss2"]
ing0_NaCl["NaCl"]
CultureMech_002460 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002460 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002460 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_KCl["KCl"]
CultureMech_002460 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NaHCO3["NaHCO3"]
CultureMech_002460 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_NH4Cl["NH4Cl"]
CultureMech_002460 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_Na2HPO4["Na2HPO4"]
CultureMech_002460 --> ing6_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing6_Na2HPO4 -.-> CHEBI_34683
ing7_Na2S2O3["Na2S2O3"]
CultureMech_002460 --> ing7_Na2S2O3
CHEBI_132112["CHEBI:132112"]
ing7_Na2S2O3 -.-> CHEBI_132112
ing8_MnO2["MnO2"]
CultureMech_002460 --> ing8_MnO2
CHEBI_136511["CHEBI:136511"]
ing8_MnO2 -.-> CHEBI_136511
ing9_Vitamin_B12["Vitamin B12"]
CultureMech_002460 --> ing9_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing9_Vitamin_B12 -.-> CHEBI_176843
ing10_Inositol["Inositol"]
CultureMech_002460 --> ing10_Inositol
CHEBI_24848["CHEBI:24848"]
ing10_Inositol -.-> CHEBI_24848
ing11_Biotin["Biotin"]
CultureMech_002460 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_Folic_acid["Folic acid"]
CultureMech_002460 --> ing12_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing12_Folic_acid -.-> CHEBI_27470
ing13_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002460 --> ing13_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing13_p_Aminobenzoic_acid -.-> CHEBI_30753
ing14_Nicotinic_acid["Nicotinic acid"]
CultureMech_002460 --> ing14_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing14_Nicotinic_acid -.-> CHEBI_15940
ing15_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
CultureMech_002460 --> ing15_DL_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing15_DL_Calcium_pantothenate -.-> CHEBI_31345
ing16_Thiamine_HCl["Thiamine HCl"]
CultureMech_002460 --> ing16_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing16_Thiamine_HCl -.-> CHEBI_49105
ing17_HCl["HCl"]
CultureMech_002460 --> ing17_HCl
CHEBI_17883["CHEBI:17883"]
ing17_HCl -.-> CHEBI_17883
ing18_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002460 --> ing18_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing18_FeCl2_x_4_H2O -.-> CHEBI_86249
ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002460 --> ing19_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
ing20_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002460 --> ing20_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing20_MnCl2_x_4_H2O -.-> CHEBI_86368
ing21_ZnCl2["ZnCl2"]
CultureMech_002460 --> ing21_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing21_ZnCl2 -.-> CHEBI_49976
ing22_H3BO3["H3BO3"]
CultureMech_002460 --> ing22_H3BO3
CHEBI_33118["CHEBI:33118"]
ing22_H3BO3 -.-> CHEBI_33118
ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002460 --> ing23_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing24_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002460 --> ing24_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing24_NiCl2_x_6_H2O -.-> CHEBI_34887
ing25_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002460 --> ing25_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing25_CuCl2_x_2_H2O -.-> CHEBI_86318
Ingredients (26)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
11.5347 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.15054 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.293255 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.391007 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
3 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
20 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 7 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
100 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 8 |
Na2S2O3
2Na.O3S2 158.111 g/mol
|
10 G_PER_L |
CHEBI:132112
sodium thiosulfate
|
|
| 9 |
MnO2
MnO2 86.936 g/mol
|
10 G_PER_L |
CHEBI:136511
manganese dioxide
|
|
| 10 | Vitamin B12 | 0.01 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 11 |
Inositol
C6H12O6 180.156 g/mol
|
0.01 G_PER_L |
CHEBI:24848
inositol
|
|
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.01 G_PER_L |
CHEBI:15956
biotin
|
|
| 13 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.01 G_PER_L |
CHEBI:27470
folic acid
|
|
| 14 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.1 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 15 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
1 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 16 |
DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
1 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 17 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
2 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 18 |
HCl
HCl 36.461 g/mol
|
10 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 19 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.48515 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 20 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.188119 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 21 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0990099 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 22 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.0693069 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 23 |
H3BO3
H3BO3 61.833 g/mol
|
0.0613861 G_PER_L |
CHEBI:33118
boric acid
|
|
| 24 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.0356436 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 25 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.0237624 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 26 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.0168317 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Preparation
- Mix components and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized):
- Adjust pH to 7.5 by bubbling with a N2-CO2 (4:1, v/v) gas mixture.
- Initially, dissolve FeCl2 x 4H2O in 25% HCl, then add distilled water followed by the other components.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- abss2 (CultureMech:002460)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.353085Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1298 |
| 2026-03-13T06:19:13.510146Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002460 |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.634563+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ABSS2" to "abss2" for consistent matching |
| 2026-06-05T07:40:46.554330+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.454985+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:52.324258+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:21.356198+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1d326b00e4101cbfc896673498f8f4bf7a34c6aebed1a765d76b3f60722c6224 |