Overview
- External ontology
- mediadive.medium:J1299 (source link unavailable) — PAENIBACILLUS DC-8A MEDIUM
- Applications
-
- Microbial cultivation
- Synonyms
-
- {'name': 'dc_8a_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:1809', 'original_category': 'bacterial'}
- pH
- 9.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1299
Composition
flowchart LR
CultureMech_002461["paenibacillus_dc_8a_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_002461 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_Urea["Urea"]
CultureMech_002461 --> ing1_Urea
CHEBI_16199["CHEBI:16199"]
ing1_Urea -.-> CHEBI_16199
ing2_Yeast_extract["Yeast extract"]
CultureMech_002461 --> ing2_Yeast_extract
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002461 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_Na2CO3["Na2CO3"]
CultureMech_002461 --> ing4_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing4_Na2CO3 -.-> CHEBI_29377
ing5_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002461 --> ing5_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing5_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing6_Resazurin["Resazurin"]
CultureMech_002461 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_Xylose["Xylose"]
CultureMech_002461 --> ing7_Xylose
CHEBI_18222["CHEBI:18222"]
ing7_Xylose -.-> CHEBI_18222
ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002461 --> ing8_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
ing9_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002461 --> ing9_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing9_FeSO4_x_7_H2O -.-> CHEBI_75836
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.9 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
Urea
CH4N2O 60.056 g/mol
|
4.2 G_PER_L |
CHEBI:16199
urea
|
|
| 3 | Yeast extract | 2 G_PER_L | ||
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
37.51 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.01, 37.5] |
| 5 |
Na2CO3
CO3.2Na 105.988 g/mol
|
1 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 6 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.0005 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
Xylose
C5H10O5 150.13 g/mol
|
10 G_PER_L |
CHEBI:18222
xylose
|
|
| 9 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
25 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 10 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.312 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Preparation
- Mix components thoroughly and adjust pH to 9.0. Distribute the medium into culture vessels (e.g., 9.0 ml in Balch tubes), replace the gas phase with N2, seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add per 900 ml the following solution (autoclaved under a N2 atmosphere):
Target organisms
No target organisms are recorded.
Provenance
Merged from 2 source records.
- dc_8a_medium (CultureMech:001246)
- paenibacillus_dc_8a_medium (CultureMech:002461)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.357997Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1299 |
| 2026-03-13T03:09:06.317582Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:13.517865Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002461 |
| 2026-03-17T06:30:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.643758+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PAENIBACILLUS DC-8A MEDIUM" to "paenibacillus_dc_8a_medium" for consistent matching |
| 2026-06-05T07:41:40.809617+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.853309+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:29.680789+00:00 | recipe-merger | Merged 2 duplicate recipes into canonical record | Sources: dc_8a_medium, paenibacillus_dc_8a_medium |
| 2026-08-06T03:05:29.680846+00:00 | merge_recipes.py | MERGED_RECIPES | merged 2 source recipe(s) on fingerprint 63089040c9d79edbcad3a14718778d4afde597559468258ad521dd704973e35b |