Overview
- External ontology
- mediadive.medium:J129 (source link unavailable) — ACIDIPHILIUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 3.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=129 Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_002462["acidiphilium_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_002462 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_KCl["KCl"]
CultureMech_002462 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_K2HPO4["K2HPO4"]
CultureMech_002462 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002462 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
CultureMech_002462 --> ing4_Pancreatic_digest_of_casein
ing5_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
CultureMech_002462 --> ing5_Peptic_digest_of_soybean_meal
ing6_Glucose["Glucose"]
CultureMech_002462 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
ing7_Sodium_chloride["Sodium chloride"]
CultureMech_002462 --> ing7_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing7_Sodium_chloride -.-> CHEBI_26710
ing8_Dipotassium_phosphate["Dipotassium phosphate"]
CultureMech_002462 --> ing8_Dipotassium_phosphate
ing8_Dipotassium_phosphate -.-> CHEBI_131527
ing9_Agar["Agar"]
CultureMech_002462 --> ing9_Agar
CHEBI_2509["CHEBI:2509"]
ing9_Agar -.-> CHEBI_2509
ing10_Glucose["Glucose"]
CultureMech_002462 --> ing10_Glucose
ing10_Glucose -.-> CHEBI_17234
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
17.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 6 |
Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
3.0 G_PER_L |
FOODON:03315720
vegetable protein, hydrolyzed
|
Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 7 |
Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:17234
glucose
|
Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 8 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 9 |
Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
2.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 10 |
Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
|
15.0 G_PER_L |
CHEBI:2509
agar
|
Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product |
| 11 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
Preparation
- Adjust pH to 3.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J129_ACIDIPHILIUM_MEDIUM (CultureMech:002462)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.779329Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J129 |
| 2026-03-13T06:19:13.522149Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002462 |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.648903+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACIDIPHILIUM MEDIUM" to "acidiphilium_medium" for consistent matching |
| 2026-06-05T07:40:16.674271+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.781914+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:35.368749+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1db773b3220e61b9a8de76cbb6e341bdf6ebe905a5eaf0cfb3320b86327c9d56 |