Overview
- External ontology
- mediadive.medium:J1301 (source link unavailable) — SPIROCHAETEA NS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1301
Composition
flowchart LR
CultureMech_002465["spirochaetea_ns_medium"]
ing0_NaNO3["NaNO3"]
CultureMech_002465 --> ing0_NaNO3
CHEBI_63005["CHEBI:63005"]
ing0_NaNO3 -.-> CHEBI_63005
ing1_K2HPO4["K2HPO4"]
CultureMech_002465 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NH4Cl["NH4Cl"]
CultureMech_002465 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002465 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002465 --> ing4_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002465 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_Resazurin["Resazurin"]
CultureMech_002465 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_Maltose["Maltose"]
CultureMech_002465 --> ing7_Maltose
CHEBI_17306["CHEBI:17306"]
ing7_Maltose -.-> CHEBI_17306
ing8_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002465 --> ing8_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_x_9_H2O -.-> CHEBI_76209
ing9_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002465 --> ing9_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing9_NiCl2_x_6_H2O -.-> CHEBI_34887
ing10_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002465 --> ing10_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing10_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing11_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002465 --> ing11_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing11_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing12_Biotin["Biotin"]
CultureMech_002465 --> ing12_Biotin
CHEBI_15956["CHEBI:15956"]
ing12_Biotin -.-> CHEBI_15956
ing13_Folic_acid["Folic acid"]
CultureMech_002465 --> ing13_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing13_Folic_acid -.-> CHEBI_27470
ing14_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002465 --> ing14_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing14_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing15_Thiamine_HCl["Thiamine HCl"]
CultureMech_002465 --> ing15_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing15_Thiamine_HCl -.-> CHEBI_49105
ing16_Riboflavin["Riboflavin"]
CultureMech_002465 --> ing16_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing16_Riboflavin -.-> CHEBI_17015
ing17_Nicotinic_acid["Nicotinic acid"]
CultureMech_002465 --> ing17_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing17_Nicotinic_acid -.-> CHEBI_15940
ing18_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002465 --> ing18_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing18_Calcium_pantothenate -.-> CHEBI_31345
ing19_Vitamin_B12["Vitamin B12"]
CultureMech_002465 --> ing19_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing19_Vitamin_B12 -.-> CHEBI_176843
ing20_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002465 --> ing20_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing20_p_Aminobenzoic_acid -.-> CHEBI_30753
ing21_Lipoic_acid["Lipoic acid"]
CultureMech_002465 --> ing21_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing21_Lipoic_acid -.-> CHEBI_16494
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaNO3
NO3.Na 84.994 g/mol
|
0.965251 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.19305 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.241313 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.3861 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.482625 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.106178 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000965251 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
Maltose
C12H22O11 342.297 g/mol
|
10 G_PER_L |
CHEBI:17306
maltose
|
|
| 9 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 10 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.03 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 11 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 12 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0004 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 13 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 14 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 15 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 16 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 17 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 18 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 19 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 20 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 21 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 22 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components thoroughly, adjust pH to 8.0, bring to a boil for a few seconds and cool down under a stream of N2. Distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under a N2 gas stream, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized):
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- spirochaetea_ns_medium (CultureMech:002465)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.363857Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1301 |
| 2026-03-13T06:19:13.538722Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002465 |
| 2026-03-17T06:30:13.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.668534+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SPIROCHAETEA NS MEDIUM" to "spirochaetea_ns_medium" for consistent matching |
| 2026-06-05T07:41:46.406591+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.665142+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:56.148244+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f412ca677a5ce902592f56acbda2d57acb6091ff4119ddaa3c9b2b90e396a197 |