Overview
- External ontology
- mediadive.medium:J1304 (source link unavailable) — FRESHWATER MEDIUM WITH RHAMNOSE
- Applications
-
- Microbial cultivation
- pH
- 6.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1304
Composition
flowchart LR
CultureMech_002468["freshwater_medium_with_rhamnose"]
ing0_NaCl["NaCl"]
CultureMech_002468 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002468 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002468 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_NH4Cl["NH4Cl"]
CultureMech_002468 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_KH2PO4["KH2PO4"]
CultureMech_002468 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_KCl["KCl"]
CultureMech_002468 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Resazurin["Resazurin"]
CultureMech_002468 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_NaHCO3["NaHCO3"]
CultureMech_002468 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_L_Rhamnose["L-Rhamnose"]
CultureMech_002468 --> ing8_L_Rhamnose
CHEBI_62345["CHEBI:62345"]
ing8_L_Rhamnose -.-> CHEBI_62345
ing9_Yeast_extract["Yeast extract"]
CultureMech_002468 --> ing9_Yeast_extract
ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002468 --> ing10_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_x_9_H2O -.-> CHEBI_76209
ing11_Na2_EDTA["Na2-EDTA"]
CultureMech_002468 --> ing11_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing11_Na2_EDTA -.-> CHEBI_64734
ing12_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002468 --> ing12_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing12_FeCl2_x_4_H2O -.-> CHEBI_86249
ing13_H3BO3["H3BO3"]
CultureMech_002468 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002468 --> ing14_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002468 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002468 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002468 --> ing17_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002468 --> ing18_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002468 --> ing19_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing20_Vitamin_B12["Vitamin B12"]
CultureMech_002468 --> ing20_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing20_Vitamin_B12 -.-> CHEBI_176843
ing21_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002468 --> ing21_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing21_p_Aminobenzoic_acid -.-> CHEBI_30753
ing22_Biotin["Biotin"]
CultureMech_002468 --> ing22_Biotin
CHEBI_15956["CHEBI:15956"]
ing22_Biotin -.-> CHEBI_15956
ing23_Nicotinic_acid["Nicotinic acid"]
CultureMech_002468 --> ing23_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing23_Nicotinic_acid -.-> CHEBI_15940
ing24_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
CultureMech_002468 --> ing24_DL_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing24_DL_Calcium_pantothenate -.-> CHEBI_31345
ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002468 --> ing25_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing26_Thiamine_HCl["Thiamine HCl"]
CultureMech_002468 --> ing26_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing26_Thiamine_HCl -.-> CHEBI_49105
ing27_NaOH["NaOH"]
CultureMech_002468 --> ing27_NaOH
CHEBI_32145["CHEBI:32145"]
ing27_NaOH -.-> CHEBI_32145
ing28_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002468 --> ing28_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing28_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing29_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002468 --> ing29_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing29_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (30)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
0.992063 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.396825 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.14881 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.248016 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.198413 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.496032 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000496032 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
30 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 | L-Rhamnose | 10 G_PER_L |
CHEBI:62345
L-rhamnose
|
|
| 10 | Yeast extract | 5 G_PER_L | ||
| 11 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 12 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
5.2 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 13 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.03 G_PER_L |
CHEBI:33118
boric acid
|
|
| 15 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 18 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 19 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.144 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 20 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 21 | Vitamin B12 | 0.05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 22 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 23 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.01 G_PER_L |
CHEBI:15956
biotin
|
|
| 24 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.1 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 25 |
DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.025 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 26 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.25 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 27 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 28 |
NaOH
HO.Na 39.997 g/mol
|
0.4 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 29 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.006 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 30 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.008 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Mix components thoroughly, adjust pH to 7.2-7.4 and autoclave under a N2-CO2 (80:20, v/v) gas mixture. After cooling, while gassing with the same gas mixture, add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized):
- Readjust pH to 7.2-7.4, if necessary. Aseptically and anaerobically distribute the medium into culture vessels (e.g., 10 ml in Hungate tubes) under the same gas mixture and seal with butyl rubber stoppers. Prior to use, add per liter the following solution (autoclaved and stored under N2):
- Dissolve EDTA x Na2 in distilled water first and adjust pH to 6.5 with NaOH. Then, proceed to add minerals.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- freshwater_medium_with_rhamnose (CultureMech:002468)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.375531Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1304 |
| 2026-03-13T06:19:13.569155Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002468 |
| 2026-03-17T06:30:19.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.702264+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "FRESHWATER MEDIUM WITH RHAMNOSE" to "freshwater_medium_with_rhamnose" for consistent matching |
| 2026-06-05T07:41:05.508664+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:58.814703+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:35.687347+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 21889617e83ad740c9a44441df33b791f331ab06522a27e2f6cf7960d4d97983 |