Overview
- External ontology
- mediadive.medium:J1307 (source link unavailable) — PPLL MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1307
Composition
flowchart LR
CultureMech_002470["ppll_medium"]
ing0_NaCl["NaCl"]
CultureMech_002470 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002470 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002470 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_Na2SO4["Na2SO4"]
CultureMech_002470 --> ing3_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing3_Na2SO4 -.-> CHEBI_32149
ing4_NH4Cl["NH4Cl"]
CultureMech_002470 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_KH2PO4["KH2PO4"]
CultureMech_002470 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_KCl["KCl"]
CultureMech_002470 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_Yeast_extract["Yeast extract"]
CultureMech_002470 --> ing7_Yeast_extract
ing8_Sodium_malate["Sodium malate"]
CultureMech_002470 --> ing8_Sodium_malate
CHEBI_91260["CHEBI:91260"]
ing8_Sodium_malate -.-> CHEBI_91260
ing9_NaHCO3["NaHCO3"]
CultureMech_002470 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002470 --> ing10_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_x_9_H2O -.-> CHEBI_76209
ing11_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002470 --> ing11_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing11_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing12_HCl["HCl"]
CultureMech_002470 --> ing12_HCl
CHEBI_17883["CHEBI:17883"]
ing12_HCl -.-> CHEBI_17883
ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002470 --> ing13_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
ing14_H3BO3["H3BO3"]
CultureMech_002470 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002470 --> ing15_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing15_MnCl2_x_4_H2O -.-> CHEBI_86368
ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002470 --> ing16_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002470 --> ing17_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002470 --> ing18_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002470 --> ing19_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002470 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing21_NaOH["NaOH"]
CultureMech_002470 --> ing21_NaOH
CHEBI_32145["CHEBI:32145"]
ing21_NaOH -.-> CHEBI_32145
ing22_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002470 --> ing22_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing22_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing23_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002470 --> ing23_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing23_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing24_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002470 --> ing24_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing24_p_Aminobenzoic_acid -.-> CHEBI_30753
ing25_Biotin["Biotin"]
CultureMech_002470 --> ing25_Biotin
CHEBI_15956["CHEBI:15956"]
ing25_Biotin -.-> CHEBI_15956
ing26_Nicotinic_acid["Nicotinic acid"]
CultureMech_002470 --> ing26_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing26_Nicotinic_acid -.-> CHEBI_15940
ing27_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
CultureMech_002470 --> ing27_DL_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing27_DL_Calcium_pantothenate -.-> CHEBI_31345
ing28_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002470 --> ing28_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing28_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing29_Sodium_phosphate_buffer["Sodium phosphate buffer"]
CultureMech_002470 --> ing29_Sodium_phosphate_buffer
CultureMech_002470 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
0.956938 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.382775 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0956938 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.82775 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.239234 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.191388 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.478469 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 8 | Yeast extract | 0.478469 G_PER_L | ||
| 9 | Sodium malate | 0.851675 G_PER_L |
CHEBI:91260
disodium malate
|
|
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
30 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 11 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 12 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 13 |
HCl
HCl 36.461 g/mol
|
12.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 14 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
2.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
0.03 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 17 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 18 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 19 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 20 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.144 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 22 |
NaOH
HO.Na 39.997 g/mol
|
0.4 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 23 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.006 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 24 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.008 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 25 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.04 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 26 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.01 G_PER_L |
CHEBI:15956
biotin
|
|
| 27 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.1 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 28 |
DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 29 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.1 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 30 | Sodium phosphate buffer | 200.0 G_PER_L | [Merged 2 duplicates: 100.0, 100.0] | |
| 31 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.1 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 32 | Vitamin B12 | 0.05 G_PER_L |
CHEBI:176843
vitamin B12
|
Preparation
- Mix components and autoclave. Cool down under a N2 gas stream. Aseptically and anaerobically add per liter the following solutions from sterile anaerobic stocks :
- Adjust pH to 7.0-7.3. Aseptically and anaerobically distribute the medium into culture vessels (e.g., 10 ml medium in Balch tubes), fill the head space with a gas mixture of N2-CO2 (4:1, v/v), and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- ppll_medium (CultureMech:002470)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.382247Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1307 |
| 2026-03-13T03:09:16.769279Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:13.586451Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002470 |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.721458+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PPLL MEDIUM" to "ppll_medium" for consistent matching |
| 2026-06-05T07:41:42.436942+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 30 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.370319+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:53.426100+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint a134eea42884c01d830be1ccb4f6ef76ac5f8df54cc256a38527c8cb0484a9ff |