Overview
- External ontology
- mediadive.medium:J1317 (source link unavailable) — RAEH MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1317
Composition
flowchart LR
CultureMech_002480["raeh_medium"]
ing0_Malic_acid["Malic acid"]
CultureMech_002480 --> ing0_Malic_acid
CHEBI_6650["CHEBI:6650"]
ing0_Malic_acid -.-> CHEBI_6650
ing1_NH4Cl["NH4Cl"]
CultureMech_002480 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002480 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002480 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_K2HPO4["K2HPO4"]
CultureMech_002480 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_KH2PO4["KH2PO4"]
CultureMech_002480 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_Yeast_extract["Yeast extract"]
CultureMech_002480 --> ing6_Yeast_extract
ing7_H3BO3["H3BO3"]
CultureMech_002480 --> ing7_H3BO3
CHEBI_33118["CHEBI:33118"]
ing7_H3BO3 -.-> CHEBI_33118
ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002480 --> ing8_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002480 --> ing9_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing10_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002480 --> ing10_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing10_CuSO4_x_5_H2O -.-> CHEBI_31440
ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002480 --> ing11_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002480 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Malic acid
C4H6O5 134.087 g/mol
|
2.48016 G_PER_L |
CHEBI:6650
malic acid
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.19048 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.198413 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0694444 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.892857 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.426587 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 | Yeast extract | 0.496032 G_PER_L | ||
| 8 |
H3BO3
H3BO3 61.833 g/mol
|
2.86 G_PER_L |
CHEBI:33118
boric acid
|
|
| 9 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.81 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 10 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.22 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 11 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.08 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 12 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.04 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.1 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Preparation
- Mix components thoroughly and adjust pH to 6.8. Bring to a boil for a seconds, cool down under a stream of N2-CO2 (80:20, v/v). Dispense the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under a H2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers and autoclave.
- Mix components thoroughly. Dilute the stock solution 1:25 to receive the trace mineral working solution.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- raeh_medium (CultureMech:002480)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.426678Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1317 |
| 2026-03-13T06:19:13.685488Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002480 |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.833459+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RAEH MEDIUM" to "raeh_medium" for consistent matching |
| 2026-06-05T07:41:43.811370+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:10.832695+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:54.299676+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3ce84f72ccad9f5bc58ce7da4000e83c873b1ceba04a9afe83f0f77134811170 |