Overview
- External ontology
- mediadive.medium:J1321 (source link unavailable) — 9K WITH SULFUR MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 2.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1321
Composition
flowchart LR
CultureMech_002485["9k_with_sulfur_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_002485 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_002485 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_KCl["KCl"]
CultureMech_002485 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002485 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Ca_NO3_2["Ca(NO3)2"]
CultureMech_002485 --> ing4_Ca_NO3_2
CHEBI_64205["CHEBI:64205"]
ing4_Ca_NO3_2 -.-> CHEBI_64205
ing5_Sulfur["Sulfur"]
CultureMech_002485 --> ing5_Sulfur
CHEBI_26833["CHEBI:26833"]
ing5_Sulfur -.-> CHEBI_26833
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
Ca(NO3)2
Ca.2NO3 164.086 g/mol
|
0.01 G_PER_L |
CHEBI:64205
calcium nitrate
|
|
| 6 |
Sulfur
S 32.067 g/mol
|
5 G_PER_L |
CHEBI:26833
sulfur atom
|
Preparation
- Mix components except sulfur, adjust pH to 2.5 with H2SO4 and autoclave. Separately sterilize sulfur by steaming for 3 hr on each of 3 successive days.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- 9k_with_sulfur_medium (CultureMech:002485)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.443266Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1321 |
| 2026-02-06T05:10:43.041110Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T06:19:13.732959Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002485 |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.885896+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "9K WITH SULFUR MEDIUM" to "9k_with_sulfur_medium" for consistent matching |
| 2026-06-05T07:40:15.990528+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:27.201496+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:42.595350+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:34.263174+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9284e6c5a5781e63f68dd6172d9ba6ebfd4dfe083a05410c4e82906bffc67c68 |