Overview
- External ontology
- mediadive.medium:J1327 (source link unavailable) — GEOBACTER NBAF MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1327
Composition
flowchart LR
CultureMech_002490["geobacter_nbaf_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002490 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_002490 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NH4Cl["NH4Cl"]
CultureMech_002490 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_KCl["KCl"]
CultureMech_002490 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NaCl["NaCl"]
CultureMech_002490 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002490 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_Fe2_SO4_3_x_n_H2O["Fe2(SO4)3 x n H2O"]
CultureMech_002490 --> ing6_Fe2_SO4_3_x_n_H2O
CHEBI_53438["CHEBI:53438"]
ing6_Fe2_SO4_3_x_n_H2O -.-> CHEBI_53438
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002490 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_Na2SeO4["Na2SeO4"]
CultureMech_002490 --> ing8_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing8_Na2SeO4 -.-> CHEBI_77775
ing9_NaHCO3["NaHCO3"]
CultureMech_002490 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Na2CO3["Na2CO3"]
CultureMech_002490 --> ing10_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing10_Na2CO3 -.-> CHEBI_29377
ing11_Sodium_acetate["Sodium acetate"]
CultureMech_002490 --> ing11_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing11_Sodium_acetate -.-> CHEBI_32954
ing12_Sodium_fumarate["Sodium fumarate"]
CultureMech_002490 --> ing12_Sodium_fumarate
CHEBI_115156["CHEBI:115156"]
ing12_Sodium_fumarate -.-> CHEBI_115156
ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002490 --> ing13_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
ing14_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002490 --> ing14_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_x_4_H2O -.-> CHEBI_86368
ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002490 --> ing15_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
ing16_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002490 --> ing16_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing16_CoCl2_x_6_H2O -.-> CHEBI_53503
ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002490 --> ing17_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002490 --> ing18_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
ing19_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002490 --> ing19_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing19_AlK_SO4_2 -.-> CHEBI_86463
ing20_H3BO3["H3BO3"]
CultureMech_002490 --> ing20_H3BO3
CHEBI_33118["CHEBI:33118"]
ing20_H3BO3 -.-> CHEBI_33118
ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002490 --> ing21_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing22_NiSO4_x_6_H2O["NiSO4 x 6 H2O"]
CultureMech_002490 --> ing22_NiSO4_x_6_H2O
CHEBI_53001["CHEBI:53001"]
ing22_NiSO4_x_6_H2O -.-> CHEBI_53001
ing23_Na2WO2_x_2_H2O["Na2WO2 x 2 H2O"]
CultureMech_002490 --> ing23_Na2WO2_x_2_H2O
ing24_Biotin["Biotin"]
CultureMech_002490 --> ing24_Biotin
CHEBI_15956["CHEBI:15956"]
ing24_Biotin -.-> CHEBI_15956
ing25_Folic_acid["Folic acid"]
CultureMech_002490 --> ing25_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing25_Folic_acid -.-> CHEBI_27470
ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002490 --> ing26_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing27_Thiamine_HCl["Thiamine HCl"]
CultureMech_002490 --> ing27_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing27_Thiamine_HCl -.-> CHEBI_49105
ing28_Riboflavin["Riboflavin"]
CultureMech_002490 --> ing28_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing28_Riboflavin -.-> CHEBI_17015
ing29_Nicotinic_acid["Nicotinic acid"]
CultureMech_002490 --> ing29_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing29_Nicotinic_acid -.-> CHEBI_15940
CultureMech_002490 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.41543 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.217606 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.197824 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.375865 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
0.356083 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0395648 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
Fe2(SO4)3 x n H2O
2Fe.3O4S 399.879 g/mol
|
0.0098912 G_PER_L |
CHEBI:53438
iron(3+) sulfate
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.098912 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
1 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
20 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 11 |
Na2CO3
CO3.2Na 105.988 g/mol
|
10 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 12 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
15 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 13 |
Sodium fumarate
C4H2O4.2Na 160.036 g/mol
|
40 G_PER_L |
CHEBI:115156
disodium fumarate
|
|
| 14 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
2.14 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 15 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 16 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.3 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 17 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.17 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 18 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.2 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 19 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.03 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 20 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.003 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 21 |
H3BO3
H3BO3 61.833 g/mol
|
5 MG_PER_L |
CHEBI:33118
boric acid
|
|
| 22 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.09 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 23 |
NiSO4 x 6 H2O
Ni.O4S 154.756 g/mol
|
0.11 G_PER_L |
CHEBI:53001
nickel sulfate
|
|
| 24 | Na2WO2 x 2 H2O | 0.02 G_PER_L | ||
| 25 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 26 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 27 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 28 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 29 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 30 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 31 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 32 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 33 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 34 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components and autoclave under a N2 atmosphere. After cooling, replace the gas phase with a N2-CO2 (4:1, v/v) gas mixture, and add the following solutions from sterile anaerobic stocks (autoclaved or *filter-sterilized).
- Check final pH to be around 7.0.
- Dissolve nitrilotriacetic acid and adjust pH to 8.0-8.5 with NaOH. Then proceed to add minerals.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- geobacter_nbaf_medium (CultureMech:002490)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:19.459318Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J1327 |
| 2026-03-13T06:19:13.771834Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002490 |
| 2026-03-17T06:30:00.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:26.929634+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GEOBACTER NBAF MEDIUM" to "geobacter_nbaf_medium" for consistent matching |
| 2026-06-05T07:41:06.136422+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.908796+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:59.017076+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:36.082933+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint db485dcd1d39ef991fdd39d9f5f3f721ac40149cd4175f1fa9e01dc03583f4e0 |