maleate_liquid_medium

CultureMech:002496 bacterial DEFINED LIQUID
Original name: MALEATE LIQUID MEDIUM

Overview

External ontology
mediadive.medium:J136 (source link unavailable) — MALEATE LIQUID MEDIUM
Applications
  • Microbial cultivation
pH
6.8
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=136

Composition

flowchart LR
    CultureMech_002496["maleate_liquid_medium"]
    ing0_Sodium_maleate["Sodium maleate"]
    CultureMech_002496 --> ing0_Sodium_maleate
    CHEBI_91263["CHEBI:91263"]
    ing0_Sodium_maleate -.-> CHEBI_91263
    ing1_NaHCO3["NaHCO3"]
    CultureMech_002496 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139
    ing2_NH4Cl["NH4Cl"]
    CultureMech_002496 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_KH2PO4["KH2PO4"]
    CultureMech_002496 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002496 --> ing4_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002496 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002496 --> ing6_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing6_Na2S_x_9_H2O -.-> CHEBI_76209
    ing7_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002496 --> ing7_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing7_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing8_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002496 --> ing8_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing8_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002496 --> ing9_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing10_ZnCl2["ZnCl2"]
    CultureMech_002496 --> ing10_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing10_ZnCl2 -.-> CHEBI_49976
    ing11_H3BO3["H3BO3"]
    CultureMech_002496 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002496 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_AlCl3["AlCl3"]
    CultureMech_002496 --> ing13_AlCl3
    CHEBI_30114["CHEBI:30114"]
    ing13_AlCl3 -.-> CHEBI_30114
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002496 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002496 --> ing15_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing15_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing16_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002496 --> ing16_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing16_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing17_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002496 --> ing17_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing17_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing18_Biotin["Biotin"]
    CultureMech_002496 --> ing18_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing18_Biotin -.-> CHEBI_15956
    ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002496 --> ing19_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing20_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002496 --> ing20_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing20_Calcium_pantothenate -.-> CHEBI_31345
    ing21_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002496 --> ing21_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing21_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing22_Nicotinamide["Nicotinamide"]
    CultureMech_002496 --> ing22_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing22_Nicotinamide -.-> CHEBI_17154
    ing23_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002496 --> ing23_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing23_Thiamine_HCl -.-> CHEBI_49105
    ing24_Lipoic_acid["Lipoic acid"]
    CultureMech_002496 --> ing24_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing24_Lipoic_acid -.-> CHEBI_16494
    ing25_Folic_acid["Folic acid"]
    CultureMech_002496 --> ing25_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing25_Folic_acid -.-> CHEBI_27470
    ing26_Vitamin_B12["Vitamin B12"]
    CultureMech_002496 --> ing26_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing26_Vitamin_B12 -.-> CHEBI_176843
    ing27_Riboflavin["Riboflavin"]
    CultureMech_002496 --> ing27_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing27_Riboflavin -.-> CHEBI_17015

Ingredients (28)

# Ingredient Concentration Ontology Notes
1 Sodium maleate
C4H2O4.2Na 160.036 g/mol
1.6 G_PER_L CHEBI:91263
disodium maleate
2 NaHCO3
CHO3.Na 84.006 g/mol
3.2 G_PER_L CHEBI:32139
sodium hydrogencarbonate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.5349 G_PER_L CHEBI:31206
ammonium chloride
4 KH2PO4
H2O4P.K 136.084 g/mol
1.4 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.0952 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.111 G_PER_L CHEBI:86158
calcium chloride dihydrate
7 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.0781 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
8 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
0.0013 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
9 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
129.8 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
10 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
125.9 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
11 ZnCl2
2Cl.Zn 136.296 g/mol
136.3 G_PER_L CHEBI:49976
zinc dichloride
12 H3BO3
H3BO3 61.833 g/mol
6.1 G_PER_L CHEBI:33118
boric acid
13 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
13 G_PER_L CHEBI:34887
nickel dichloride
14 AlCl3
AlCl3 133.341 g/mol
13.3 G_PER_L CHEBI:30114
aluminium trichloride
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
20.6 G_PER_L CHEBI:75213
sodium molybdate dihydrate
16 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
1.7 G_PER_L CHEBI:131361
disodium selenite pentahydrate
17 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
2.9 G_PER_L CHEBI:63939
sodium tungstate dihydrate
18 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
1.3 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
19 Biotin
C10H16N2O3S 244.316 g/mol
24.4 G_PER_L CHEBI:15956
biotin
20 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
13.7 G_PER_L CHEBI:30753
4-aminobenzoic acid
21 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
47.7 G_PER_L CHEBI:31345
Calcium pantothenate
22 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
16.9 G_PER_L CHEBI:30961
pyridoxine hydrochloride
23 Nicotinamide
C6H6N2O 122.127 g/mol
12.2 G_PER_L CHEBI:17154
nicotinamide
24 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
33.7 G_PER_L CHEBI:49105
thiamine hydrochloride
25 Lipoic acid
C8H14O2S2 206.332 g/mol
20.6 G_PER_L CHEBI:16494
lipoic acid
26 Folic acid
C19H19N7O6 441.404 g/mol
44.1 G_PER_L CHEBI:27470
folic acid
27 Vitamin B12 135.5 G_PER_L CHEBI:176843
vitamin B12
28 Riboflavin
C17H20N4O6 376.369 g/mol
37.6 G_PER_L CHEBI:17015
riboflavin

Preparation

  1. Adjust pH to 6.7-6.8.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.794831Z mediadive-import Imported from MediaDive Source: JCM, ID: J136
2026-03-13T06:19:13.820316Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002496
2026-03-17T06:30:37.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:26.985399+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MALEATE LIQUID MEDIUM" to "maleate_liquid_medium" for consistent matching
2026-06-05T07:41:12.606769+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 28 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.820628+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:41.718122+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 817e900da589cff15c3543b691bd4374b564c167c2c73f7c73706374864ca2ce