Overview
- External ontology
- mediadive.medium:J146 (source link unavailable) — MODIFIED OTI MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=146
Composition
flowchart LR
CultureMech_002505["modified_oti_medium"]
ing0_Polypeptone["Polypeptone"]
CultureMech_002505 --> ing0_Polypeptone
ing1_Heart_Infusion_Broth["Heart Infusion Broth"]
CultureMech_002505 --> ing1_Heart_Infusion_Broth
ing2_Yeast_extract["Yeast extract"]
CultureMech_002505 --> ing2_Yeast_extract
ing3_Pectin["Pectin"]
CultureMech_002505 --> ing3_Pectin
CHEBI_17309["CHEBI:17309"]
ing3_Pectin -.-> CHEBI_17309
ing4_Glucose["Glucose"]
CultureMech_002505 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Starch["Starch"]
CultureMech_002505 --> ing5_Starch
CHEBI_28017["CHEBI:28017"]
ing5_Starch -.-> CHEBI_28017
ing6_Sucrose["Sucrose"]
CultureMech_002505 --> ing6_Sucrose
CHEBI_17992["CHEBI:17992"]
ing6_Sucrose -.-> CHEBI_17992
ing7_Maltose["Maltose"]
CultureMech_002505 --> ing7_Maltose
CHEBI_17306["CHEBI:17306"]
ing7_Maltose -.-> CHEBI_17306
ing8_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002505 --> ing8_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing8_Sodium_pyruvate -.-> CHEBI_50144
ing9_Ribose["Ribose"]
CultureMech_002505 --> ing9_Ribose
CHEBI_33942["CHEBI:33942"]
ing9_Ribose -.-> CHEBI_33942
ing10_K2HPO4["K2HPO4"]
CultureMech_002505 --> ing10_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing10_K2HPO4 -.-> CHEBI_131527
ing11_NaCl["NaCl"]
CultureMech_002505 --> ing11_NaCl
CHEBI_26710["CHEBI:26710"]
ing11_NaCl -.-> CHEBI_26710
ing12_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002505 --> ing12_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing12_MgSO4_x_7_H2O -.-> CHEBI_31795
ing13_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002505 --> ing13_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing13_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing14_Clarified_rumen_fluid["Clarified rumen fluid"]
CultureMech_002505 --> ing14_Clarified_rumen_fluid
ing15_Agar["Agar"]
CultureMech_002505 --> ing15_Agar
CHEBI_2509["CHEBI:2509"]
ing15_Agar -.-> CHEBI_2509
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Polypeptone | 5 G_PER_L | ||
| 2 | Heart Infusion Broth | 5 G_PER_L | ||
| 3 | Yeast extract | 5 G_PER_L | ||
| 4 | Pectin | 0.8 G_PER_L |
CHEBI:17309
pectin
|
|
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
0.8 G_PER_L |
CHEBI:17234
glucose
|
|
| 6 | Starch | 0.8 G_PER_L |
CHEBI:28017
starch
|
|
| 7 |
Sucrose
C12H22O11 342.297 g/mol
|
0.8 G_PER_L |
CHEBI:17992
sucrose
|
|
| 8 |
Maltose
C12H22O11 342.297 g/mol
|
0.8 G_PER_L |
CHEBI:17306
maltose
|
|
| 9 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.8 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 10 |
Ribose
C5H10O5 150.13 g/mol
|
0.8 G_PER_L |
CHEBI:33942
ribose
|
|
| 11 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 12 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 13 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 14 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.68 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 15 | Clarified rumen fluid | 500 G_PER_L | ||
| 16 | Agar | 1.6 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 7.0. Prepare medium anaerobically and dispense into rubber-stoppered tubes under oxygen-free N2. Add inactivated sterile rabbit serum to give final concentration in each tube of 5%. Add filter-sterilized thiamine pyrophosphate to each tube to give a final concentration of 0.75 mg/100 ml. Subculture with a Pasteur pipette (20-30 drops). Gas phase for culture system should be oxygen-free N2 or N2-CO2 (90:10).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_oti_medium (CultureMech:002505)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.815824Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J146 |
| 2026-03-13T06:19:13.867930Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002505 |
| 2026-03-17T06:29:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.037608+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED OTI MEDIUM" to "modified_oti_medium" for consistent matching |
| 2026-06-05T07:41:37.558266+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.892481+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:49.457484+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 53f9462079712d4c02549b5bdc30d39a015ae1202098931eb558ba78c07bd115 |