Overview
- External ontology
- mediadive.medium:J14 (source link unavailable) — EG MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.7
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=14
Composition
flowchart LR
CultureMech_002508["eg_medium"]
ing0_Lab_Lemco_beef_extract["Lab-Lemco beef extract"]
CultureMech_002508 --> ing0_Lab_Lemco_beef_extract
ing1_Proteose_peptone_no__3["Proteose peptone no. 3"]
CultureMech_002508 --> ing1_Proteose_peptone_no__3
ing2_Yeast_extract["Yeast extract"]
CultureMech_002508 --> ing2_Yeast_extract
ing3_Na2HPO4["Na2HPO4"]
CultureMech_002508 --> ing3_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing3_Na2HPO4 -.-> CHEBI_34683
ing4_Glucose["Glucose"]
CultureMech_002508 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Starch["Starch"]
CultureMech_002508 --> ing5_Starch
CHEBI_28017["CHEBI:28017"]
ing5_Starch -.-> CHEBI_28017
ing6_L_Cystine["L-Cystine"]
CultureMech_002508 --> ing6_L_Cystine
CHEBI_16283["CHEBI:16283"]
ing6_L_Cystine -.-> CHEBI_16283
ing7_Agar["Agar"]
CultureMech_002508 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002508 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_Horse_blood["Horse blood"]
CultureMech_002508 --> ing9_Horse_blood
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Lab-Lemco beef extract | 2.4 G_PER_L | ||
| 2 | Proteose peptone no. 3 | 10 G_PER_L | ||
| 3 | Yeast extract | 5 G_PER_L | ||
| 4 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
4 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
1.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 6 | Starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
|
| 7 |
L-Cystine
C6H12N2O4S2 240.306 g/mol
|
0.2 G_PER_L |
CHEBI:16283
L-cystine
|
|
| 8 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 | Horse blood | 50 G_PER_L |
Preparation
- Add L-cystine to 50 ml of 1 N HCl and mix thoroughly. Add the remaining components and bring volume to 950 ml. Adjust pH to 7.6-7.8. After autoclaving and then cooling to 50C, aseptically add 50.0 ml of horse blood. Mix thoroughly and pour into sterile petri dishes or distribute into sterile tubes.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- eg_medium (CultureMech:002508)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.582513Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J14 |
| 2026-03-13T06:19:13.883834Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002508 |
| 2026-03-17T06:30:32.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.055261+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "EG MEDIUM" to "eg_medium" for consistent matching |
| 2026-06-05T07:40:59.411922+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:33.432161+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint dd848cc732a5ba8547b663a1fb2f9bdda21ee86a0edde1336da6e5a259d6bfaf |