Overview
- External ontology
- mediadive.medium:J155 (source link unavailable) — DUBOS SALTS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=155
Composition
flowchart LR
CultureMech_002513["dubos_salts_medium"]
ing0_NaNO3["NaNO3"]
CultureMech_002513 --> ing0_NaNO3
CHEBI_63005["CHEBI:63005"]
ing0_NaNO3 -.-> CHEBI_63005
ing1_K2HPO4["K2HPO4"]
CultureMech_002513 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002513 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_KCl["KCl"]
CultureMech_002513 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002513 --> ing4_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing4_FeSO4_x_7_H2O -.-> CHEBI_75836
ing5_Agar["Agar"]
CultureMech_002513 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaNO3
NO3.Na 84.994 g/mol
|
0.5 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.01 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 6 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 7.2. Autoclave and dispense into slopes. When the agar has solidified, place a strip of sterile filter paper onto the surface of each slope. Inoculate onto the filter paper.
Target organisms
No target organisms are recorded.
Provenance
Merged from 3 source records.
- JCM_J155_DUBOS_SALTS_MEDIUM (CultureMech:002513)
- KOMODO_1161_DUBOS_SALTS_MEDIUM (CultureMech:003872)
- dubos_salts_medium (CultureMech:000601)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.836517Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J155 |
| 2026-03-13T06:19:13.909464Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002513 |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.084131+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DUBOS SALTS MEDIUM" to "dubos_salts_medium" for consistent matching |
| 2026-06-05T07:40:16.721039+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.794789+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:35.758671+00:00 | recipe-merger | Merged 3 duplicate recipes into canonical record | Sources: JCM_J155_DUBOS_SALTS_MEDIUM, KOMODO_1161_DUBOS_SALTS_MEDIUM, dubos_salts_medium |
| 2026-08-06T03:04:35.758714+00:00 | merge_recipes.py | MERGED_RECIPES | merged 3 source recipe(s) on fingerprint d330473c472e3e2e009e0386086fbd9233eafc6e6da5184020e328bbcc42ab2e |