thermococcus_stetteri_medium

Overview

External ontology

mediadive.medium:J160 (source link unavailable) — THERMOCOCCUS STETTERI MEDIUM

Applications

• Microbial cultivation

Synonyms

• {'name': 'thermococcus_litoralis_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J161', 'original_category': 'archaea'}
• {'name': 'thermococcus_fumicolans_medium', 'source': 'MediaDive', 'source_id': 'mediadive.medium:J241', 'original_category': 'archaea'}

pH

6.5

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=160

Composition

flowchart LR
    CultureMech_002519["thermococcus_stetteri_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002519 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_002519 --> ing1_Yeast_extract
    ing2_Bacto_peptone["Bacto peptone"]
    CultureMech_002519 --> ing2_Bacto_peptone
    ing3_Sulfur["Sulfur"]
    CultureMech_002519 --> ing3_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing3_Sulfur -.-> CHEBI_26833
    ing4_Resazurin["Resazurin"]
    CultureMech_002519 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002519 --> ing5__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing5__NH4_2SO4 -.-> CHEBI_62946
    ing6_KH2PO4["KH2PO4"]
    CultureMech_002519 --> ing6_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing6_KH2PO4 -.-> CHEBI_63036
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002519 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002519 --> ing8_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing9_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_002519 --> ing9_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing9_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002519 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_002519 --> ing11_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing11_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002519 --> ing12_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002519 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002519 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_VOSO4_x_n_H2O["VOSO4 x n H2O"]
    CultureMech_002519 --> ing15_VOSO4_x_n_H2O
    CHEBI_87020["CHEBI:87020"]
    ing15_VOSO4_x_n_H2O -.-> CHEBI_87020
    ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002519 --> ing16_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing16_CoSO4_x_7_H2O -.-> CHEBI_91244

Ingredients (17)

Preparation

1. Mix ingredients, except yeast extract, sulfur and Na2S x 9H2O, adjust pH to 6.5 with H2SO4, and autoclave under a N2 atmosphere. Separately autoclave yeast extract and peptone as 10% solutions under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S x 9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add the yeast extract and peptone solutions, and sulfur to the medium. Prior to inoculation, add the sterile Na2S x 9H2O solution, and readjust pH to 6.5, if necessary.

Target organisms

No target organisms are recorded.

Provenance

Merged from 3 source records.

• JCM_J161_THERMOCOCCUS_LITORALIS_MEDIUM (CultureMech:002520)
• thermococcus_fumicolans_medium (CultureMech:002602)
• thermococcus_stetteri_medium (CultureMech:002519)