Overview
- External ontology
- mediadive.medium:J164 (source link unavailable) — HYDROGENOBACTER ACIDOPHILUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 3.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=164
Composition
flowchart LR
CultureMech_002523["hydrogenobacter_acidophilus_medium"]
ing0_Sulfur["Sulfur"]
CultureMech_002523 --> ing0_Sulfur
CHEBI_26833["CHEBI:26833"]
ing0_Sulfur -.-> CHEBI_26833
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_002523 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_K2HPO4["K2HPO4"]
CultureMech_002523 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_NaCl["NaCl"]
CultureMech_002523 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002523 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002523 --> ing5_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
ing6_CaCl2["CaCl2"]
CultureMech_002523 --> ing6_CaCl2
CHEBI_3312["CHEBI:3312"]
ing6_CaCl2 -.-> CHEBI_3312
ing7_NiSO4_x_6_H2O["NiSO4 x 6 H2O"]
CultureMech_002523 --> ing7_NiSO4_x_6_H2O
CHEBI_53001["CHEBI:53001"]
ing7_NiSO4_x_6_H2O -.-> CHEBI_53001
ing8_MoO3["MoO3"]
CultureMech_002523 --> ing8_MoO3
CHEBI_30627["CHEBI:30627"]
ing8_MoO3 -.-> CHEBI_30627
ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002523 --> ing9_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing10_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002523 --> ing10_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing10_CuSO4_x_5_H2O -.-> CHEBI_31440
ing11_H3BO3["H3BO3"]
CultureMech_002523 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002523 --> ing12_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing12_MnSO4_x_n_H2O -.-> CHEBI_86360
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002523 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Sulfur
S 32.067 g/mol
|
4.99002 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.998004 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.998004 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.998004 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.299401 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.000998004 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 7 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.000998004 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 8 |
NiSO4 x 6 H2O
Ni.O4S 154.756 g/mol
|
5.98802e-05 G_PER_L |
CHEBI:53001
nickel sulfate
|
|
| 9 |
MoO3
MoO3 143.937 g/mol
|
0.001 G_PER_L |
CHEBI:30627
molybdenum trioxide
|
|
| 10 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.007 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 11 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.0005 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.001 G_PER_L |
CHEBI:33118
boric acid
|
|
| 13 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.001 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.001 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
Preparation
- Adjust pH to 3.0 with HCl.
- Sterilize sulfur separately.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J164_HYDROGENOBACTER_ACIDOPHILUS_MEDIUM (CultureMech:002523)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.854981Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J164 |
| 2026-03-13T06:19:13.950303Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002523 |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.130636+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HYDROGENOBACTER ACIDOPHILUS MEDIUM" to "hydrogenobacter_acidophilus_medium" for consistent matching |
| 2026-06-05T07:40:16.751174+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.805962+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:35.767812+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0e74ee7c3c5de5da9e0452fe8c324b57dfc86937fe5e15a3dcf25e3c4bb7a4d7 |