Overview
- External ontology
- mediadive.medium:J18 (source link unavailable) — LACTIC ACID BACTERIA BROTH
- Applications
-
- Microbial cultivation
- pH
- 5.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=18
Composition
flowchart LR
CultureMech_002551["lactic_acid_bacteria_broth"]
ing0_Trypticase_peptone["Trypticase peptone"]
CultureMech_002551 --> ing0_Trypticase_peptone
ing1_Yeast_autolysate["Yeast autolysate"]
CultureMech_002551 --> ing1_Yeast_autolysate
ing2_Sodium_acetate["Sodium acetate"]
CultureMech_002551 --> ing2_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing2_Sodium_acetate -.-> CHEBI_32954
ing3_Glucose["Glucose"]
CultureMech_002551 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_K2HPO4["K2HPO4"]
CultureMech_002551 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_KH2PO4["KH2PO4"]
CultureMech_002551 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002551 --> ing6_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
ing7_NaCl["NaCl"]
CultureMech_002551 --> ing7_NaCl
CHEBI_26710["CHEBI:26710"]
ing7_NaCl -.-> CHEBI_26710
ing8_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002551 --> ing8_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing8_FeSO4_x_7_H2O -.-> CHEBI_75836
ing9_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002551 --> ing9_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing9_MnSO4_x_n_H2O -.-> CHEBI_86360
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Trypticase peptone | 9.90099 G_PER_L | ||
| 2 | Yeast autolysate | 4.9505 G_PER_L | ||
| 3 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
11.8812 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
9.90099 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
50 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
50 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 8 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 9 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 10 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
1 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Preparation
- Adjust pH to 5.1-5.3.
- <I>Solution B: </I>
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- lactic_acid_bacteria_broth (CultureMech:002551)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.591057Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J18 |
| 2026-03-13T06:19:14.099536Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002551 |
| 2026-03-17T06:30:37.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.300467+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LACTIC ACID BACTERIA BROTH" to "lactic_acid_bacteria_broth" for consistent matching |
| 2026-06-05T07:41:10.687635+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.464203+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:39.869847+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0c87ce316fc67efb1274377fe2d0b17b04438d225799a9fda113a263c11faf13 |