Overview
- External ontology
- mediadive.medium:J192 (source link unavailable) — PYS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=192
Composition
flowchart LR
CultureMech_002554["pys_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002554 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_002554 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_NaCl["NaCl"]
CultureMech_002554 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002554 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002554 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_002554 --> ing5_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing5_Sodium_pyruvate -.-> CHEBI_50144
ing6_Yeast_extract["Yeast extract"]
CultureMech_002554 --> ing6_Yeast_extract
ing7_Na2_EDTA["Na2-EDTA"]
CultureMech_002554 --> ing7_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing7_Na2_EDTA -.-> CHEBI_64734
ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_002554 --> ing8_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
ing9_ZnCl2["ZnCl2"]
CultureMech_002554 --> ing9_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing9_ZnCl2 -.-> CHEBI_49976
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002554 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_H3BO3["H3BO3"]
CultureMech_002554 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002554 --> ing12_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002554 --> ing13_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002554 --> ing14_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002554 --> ing15_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.999001 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.999001 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
0.1998 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.1998 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.04995 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
2.1978 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 7 | Yeast extract | 0.999001 G_PER_L | ||
| 8 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
5.2 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
|
| 9 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 10 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.062 G_PER_L |
CHEBI:33118
boric acid
|
|
| 13 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 14 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.017 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 15 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 16 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust pH to 6.8.
- Separately sterilize sodium pyruvate by filtration, and aseptically add to the medium.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J192_PYS_MEDIUM (CultureMech:002554)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.920425Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J192 |
| 2026-03-13T06:19:14.116250Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002554 |
| 2026-03-17T06:30:33.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.320172+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYS MEDIUM" to "pys_medium" for consistent matching |
| 2026-06-05T07:40:16.811792+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.826984+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:35.786183+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0f622cc67447a635adfb6ba6305e4b0f7263ed8b2e4fed28bef35762a84d9d09 |