thermoproteus_tenax_medium

CultureMech:002558 archaea COMPLEX LIQUID
Original name: THERMOPROTEUS TENAX MEDIUM

Overview

External ontology
mediadive.medium:J196 (source link unavailable) — THERMOPROTEUS TENAX MEDIUM
Applications
  • Microbial cultivation
pH
5.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=196

Composition

flowchart LR
    CultureMech_002558["thermoproteus_tenax_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_002558 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002558 --> ing1_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing1_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002558 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
    CultureMech_002558 --> ing3_CaSO4_x_2_H2O
    CHEBI_32583["CHEBI:32583"]
    ing3_CaSO4_x_2_H2O -.-> CHEBI_32583
    ing4_KH2PO3["KH2PO3"]
    CultureMech_002558 --> ing4_KH2PO3
    ing5_NaF["NaF"]
    CultureMech_002558 --> ing5_NaF
    CHEBI_28741["CHEBI:28741"]
    ing5_NaF -.-> CHEBI_28741
    ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002558 --> ing6_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing7_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
    CultureMech_002558 --> ing7_Na2B4O7_x_10_H2O
    CHEBI_131366["CHEBI:131366"]
    ing7_Na2B4O7_x_10_H2O -.-> CHEBI_131366
    ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002558 --> ing8_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing9_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002558 --> ing9_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing9_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing10_Na2MoO7_x_2_H2O["Na2MoO7 x 2 H2O"]
    CultureMech_002558 --> ing10_Na2MoO7_x_2_H2O
    ing11_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002558 --> ing11_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing11_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing12_Yeast_extract["Yeast extract"]
    CultureMech_002558 --> ing12_Yeast_extract
    ing13_Starch["Starch"]
    CultureMech_002558 --> ing13_Starch
    CHEBI_28017["CHEBI:28017"]
    ing13_Starch -.-> CHEBI_28017
    ing14_Sulfur["Sulfur"]
    CultureMech_002558 --> ing14_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing14_Sulfur -.-> CHEBI_26833
    ing15_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002558 --> ing15_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing15_Na2S_x_9_H2O -.-> CHEBI_76209
    ing16_Resazurin["Resazurin"]
    CultureMech_002558 --> ing16_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing16_Resazurin -.-> CHEBI_8806

Ingredients (17)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.264 G_PER_L CHEBI:62946
ammonium sulfate
2 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.556 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.492 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
0.344 G_PER_L CHEBI:32583
calcium sulfate dihydrate
5 KH2PO3 0.014 G_PER_L
6 NaF
F.Na 41.988 g/mol
0.00084 G_PER_L CHEBI:28741
sodium fluoride
7 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.00018 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
8 Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
0.00045 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
9 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
22 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
10 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
5 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
11 Na2MoO7 x 2 H2O 3 G_PER_L
12 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
13 Yeast extract 0.2 G_PER_L
14 Starch 5 G_PER_L CHEBI:28017
starch
15 Sulfur
S 32.067 g/mol
10 G_PER_L CHEBI:26833
sulfur atom
16 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.5 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
17 Resazurin
C12H7NO4 229.191 g/mol
0.001 G_PER_L CHEBI:8806
Resazurin

Preparation

  1. Mix ingredients, except yeast extract, starch, sulfur and Na2S x 9H2O, and filter-sterilize while gassing with N2. Autoclave yeast extract and starch as 10% solutions and Na2S x 9H2O as a 5% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Aseptically and anaerobically add the yeast extract and starch solutions, and sulfur to the medium. Prior to inoculation, add the sterile Na2S x 9H2O solution, and adjust pH to 5.5 with sterile 1 N H2SO4.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.932142Z mediadive-import Imported from MediaDive Source: JCM, ID: J196
2026-03-13T06:19:14.147809Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002558
2026-03-17T06:30:08.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.357187+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "THERMOPROTEUS TENAX MEDIUM" to "thermoproteus_tenax_medium" for consistent matching
2026-06-05T07:41:50.473420+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:13.138103+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:33.023749+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint bda77c802eeeed3764f4bf451e8e758e50acea728235218ec8559c8ab25c6204