jcm_medium_no_198

CultureMech:002560 bacterial DEFINED LIQUID
Original name: JCM MEDIUM No. 198

Overview

External ontology
mediadive.medium:J198 (source link unavailable) — JCM MEDIUM No. 198
Applications
  • Microbial cultivation
pH
7.4
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=198

Composition

flowchart LR
    CultureMech_002560["jcm_medium_no_198"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002560 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_002560 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002560 --> ing2_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing2_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing3_NH4Cl["NH4Cl"]
    CultureMech_002560 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_NaHCO3["NaHCO3"]
    CultureMech_002560 --> ing4_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing4_NaHCO3 -.-> CHEBI_32139
    ing5_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002560 --> ing5_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing5_Na2S_x_9_H2O -.-> CHEBI_76209
    ing6_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002560 --> ing6_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing6_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing7_Resazurin["Resazurin"]
    CultureMech_002560 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002560 --> ing8_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing8_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002560 --> ing9_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002560 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_ZnCl2["ZnCl2"]
    CultureMech_002560 --> ing11_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing11_ZnCl2 -.-> CHEBI_49976
    ing12_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002560 --> ing12_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing12_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing13_H3BO3["H3BO3"]
    CultureMech_002560 --> ing13_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing13_H3BO3 -.-> CHEBI_33118
    ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002560 --> ing14_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002560 --> ing15_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing16_Biotin["Biotin"]
    CultureMech_002560 --> ing16_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing16_Biotin -.-> CHEBI_15956
    ing17_Folic_acid["Folic acid"]
    CultureMech_002560 --> ing17_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing17_Folic_acid -.-> CHEBI_27470
    ing18_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002560 --> ing18_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing18_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing19_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002560 --> ing19_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing19_Thiamine_HCl -.-> CHEBI_49105
    ing20_Riboflavin["Riboflavin"]
    CultureMech_002560 --> ing20_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing20_Riboflavin -.-> CHEBI_17015
    ing21_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002560 --> ing21_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing21_Nicotinic_acid -.-> CHEBI_15940
    ing22_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002560 --> ing22_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing22_Calcium_pantothenate -.-> CHEBI_31345
    ing23_Vitamin_B12["Vitamin B12"]
    CultureMech_002560 --> ing23_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing23_Vitamin_B12 -.-> CHEBI_176843
    ing24_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002560 --> ing24_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing24_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing25_Lipoic_acid["Lipoic acid"]
    CultureMech_002560 --> ing25_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing25_Lipoic_acid -.-> CHEBI_16494

Ingredients (26)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.736016 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.736016 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.453288 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 [Merged 2 duplicates: 0.353288, 0.1]
4 NH4Cl
Cl.H4N 53.492 g/mol
0.981354 G_PER_L CHEBI:31206
ammonium chloride
5 NaHCO3
CHO3.Na 84.006 g/mol
3.92542 G_PER_L CHEBI:32139
sodium hydrogencarbonate
6 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.490677 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
7 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.490677 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
8 Resazurin
C12H7NO4 229.191 g/mol
0.000981354 G_PER_L CHEBI:8806
Resazurin
9 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
4.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
10 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
0.4 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.17 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
13 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.02 G_PER_L CHEBI:86158
calcium chloride dihydrate
14 H3BO3
H3BO3 61.833 g/mol
0.019 G_PER_L CHEBI:33118
boric acid
15 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
16 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.03 G_PER_L CHEBI:34887
nickel dichloride
17 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
18 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
19 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
20 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
21 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
22 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
23 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
24 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
25 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
26 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix ingredients, except NaHCO3, Trace vitamins, Na2S x 9H2O and L-cysteine x HCl x H2O, and autoclave under a H2-CO2 (80:20) atmosphere. Filter-sterilize NaHCO3 as 8% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S x 9H2O and L-cysteine x HCl x H2O as 5% solutions under a N2 atmosphere. Aseptically and anaerobically add the NaHCO3 solution and Trace vitamins to the medium under a H2-CO2 (80:20) atmosphere. Prior to inoculation, add sterile the Na2S x 9H2O and L-cysteine x HCl solutions, and adjust pH to 7.4. Pressurize inoculated bottles to 200 kPa H2-CO2 (80:20).
  2. Adjust pH to 7.0 with 1 N KOH.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:15.940861Z mediadive-import Imported from MediaDive Source: JCM, ID: J198
2026-03-13T03:09:37.802584Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:14.172538Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002560
2026-03-17T06:30:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.384823+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "JCM MEDIUM No. 198" to "jcm_medium_no_198" for consistent matching
2026-06-05T07:41:09.902699+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 26 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:00.215215+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:39.063153+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 236d77fbf04305e7f637f76c30a434719c10bc5c91e3ab6e4037b1938150776a