Overview
- External ontology
- mediadive.medium:J215 (source link unavailable) — PYROBACULUM AEROPHILUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=215
Composition
flowchart LR
CultureMech_002577["pyrobaculum_aerophilum_medium"]
ing0_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_002577 --> ing0_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing0_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
ing1_NH4Cl["NH4Cl"]
CultureMech_002577 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
CultureMech_002577 --> ing2__NH4_2Ni_SO4_2_x_6_H2O
CHEBI_86149["CHEBI:86149"]
ing2__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
ing3_Na2SeO4["Na2SeO4"]
CultureMech_002577 --> ing3_Na2SeO4
CHEBI_77775["CHEBI:77775"]
ing3_Na2SeO4 -.-> CHEBI_77775
ing4_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_002577 --> ing4_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing4_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing5_NaHCO3["NaHCO3"]
CultureMech_002577 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_KH2PO4["KH2PO4"]
CultureMech_002577 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7_Yeast_extract["Yeast extract"]
CultureMech_002577 --> ing7_Yeast_extract
ing8_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
CultureMech_002577 --> ing8_Na2S2O3_x_5_H2O
CHEBI_32150["CHEBI:32150"]
ing8_Na2S2O3_x_5_H2O -.-> CHEBI_32150
ing9_NaCl["NaCl"]
CultureMech_002577 --> ing9_NaCl
CHEBI_26710["CHEBI:26710"]
ing9_NaCl -.-> CHEBI_26710
ing10_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002577 --> ing10_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing10_MgCl2_x_6_H2O -.-> CHEBI_86345
ing11_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002577 --> ing11_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing11_MgSO4_x_7_H2O -.-> CHEBI_31795
ing12_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002577 --> ing12_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing12_CaCl2_x_2_H2O -.-> CHEBI_86158
ing13_Na2SO4["Na2SO4"]
CultureMech_002577 --> ing13_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing13_Na2SO4 -.-> CHEBI_32149
ing14_Na2CO3["Na2CO3"]
CultureMech_002577 --> ing14_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing14_Na2CO3 -.-> CHEBI_29377
ing15_NaBr["NaBr"]
CultureMech_002577 --> ing15_NaBr
CHEBI_63004["CHEBI:63004"]
ing15_NaBr -.-> CHEBI_63004
ing16_KBr["KBr"]
CultureMech_002577 --> ing16_KBr
CHEBI_32030["CHEBI:32030"]
ing16_KBr -.-> CHEBI_32030
ing17_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
CultureMech_002577 --> ing17_SrCl2_x_6_H2O
CHEBI_36385["CHEBI:36385"]
ing17_SrCl2_x_6_H2O -.-> CHEBI_36385
ing18_H3BO3["H3BO3"]
CultureMech_002577 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_Na2HPO4["Na2HPO4"]
CultureMech_002577 --> ing19_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing19_Na2HPO4 -.-> CHEBI_34683
ing20_NaF["NaF"]
CultureMech_002577 --> ing20_NaF
CHEBI_28741["CHEBI:28741"]
ing20_NaF -.-> CHEBI_28741
ing21_Sodium_silicate["Sodium silicate"]
CultureMech_002577 --> ing21_Sodium_silicate
CHEBI_60720["CHEBI:60720"]
ing21_Sodium_silicate -.-> CHEBI_60720
ing22_KI["KI"]
CultureMech_002577 --> ing22_KI
CHEBI_8346["CHEBI:8346"]
ing22_KI -.-> CHEBI_8346
ing23_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002577 --> ing23_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing23_Nitrilotriacetic_acid -.-> CHEBI_44557
ing24_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002577 --> ing24_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing24_MnSO4_x_n_H2O -.-> CHEBI_86360
ing25_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002577 --> ing25_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing25_FeSO4_x_7_H2O -.-> CHEBI_75836
ing26_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002577 --> ing26_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing26_CoSO4_x_7_H2O -.-> CHEBI_91244
ing27_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002577 --> ing27_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing27_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing28_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_002577 --> ing28_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing28_CuSO4_x_5_H2O -.-> CHEBI_31440
ing29_AlK_SO4_2["AlK(SO4)2"]
CultureMech_002577 --> ing29_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing29_AlK_SO4_2 -.-> CHEBI_86463
CultureMech_002577 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.002 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
(NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
|
0.002 G_PER_L |
CHEBI:86149
ammonium nickel sulfate hexahydrate
|
|
| 4 |
Na2SeO4
2Na.O4Se 188.936 g/mol
|
0.0001 G_PER_L |
CHEBI:77775
sodium selenate
|
|
| 5 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.0001 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.07 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 8 | Yeast extract | 0.5 G_PER_L | ||
| 9 |
Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
|
| 10 |
NaCl
Cl.Na 58.443 g/mol
|
48.15 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 47.15, 1.0] |
| 11 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
18.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 12 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
10.0 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 7.0, 3.0] |
| 13 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
3.23 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 3.13, 0.1] |
| 14 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.24 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 15 |
Na2CO3
CO3.2Na 105.988 g/mol
|
0.1 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 16 |
NaBr
Br.Na 102.894 g/mol
|
0.1 G_PER_L |
CHEBI:63004
sodium bromide
|
|
| 17 |
KBr
Br.K 119.002 g/mol
|
0.08 G_PER_L |
CHEBI:32030
potassium bromide
|
|
| 18 |
SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.072 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.062 G_PER_L |
CHEBI:33118
boric acid
|
[Merged 2 duplicates: 0.052, 0.01] |
| 20 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
0.0081 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 21 |
NaF
F.Na 41.988 g/mol
|
0.0024 G_PER_L |
CHEBI:28741
sodium fluoride
|
|
| 22 |
Sodium silicate
2Na.O3Si 122.063 g/mol
|
0.0004 G_PER_L |
CHEBI:60720
sodium silicate
|
|
| 23 |
KI
I.K 166.002 g/mol
|
5e-05 G_PER_L |
CHEBI:8346
potassium iodide
|
|
| 24 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 25 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 26 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 27 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 28 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 29 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 30 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 31 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Adjust pH to 7.0 with 1 N H2SO4, and sterilize by filtration. Dispense the medium into bottles under a N2-CO2-O2 (80:20:1) atmosphere. Pressurize inoculated bottles to 200 kPa N2-CO2-O2 (80:20:1).
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pyrobaculum_aerophilum_medium (CultureMech:002577)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.982301Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J215 |
| 2026-01-28T19:48:13.165839Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:08:49.944191Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:14.274346Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002577 |
| 2026-03-17T06:29:53.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.498907+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PYROBACULUM AEROPHILUM MEDIUM" to "pyrobaculum_aerophilum_medium" for consistent matching |
| 2026-06-05T07:41:43.185199+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.762989+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:10.623151+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 10 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:32.329734+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint bc93f572c0f4e93733b3ea65fe6cf45067eac9f86b7616cca60b27384a76926f |