Overview
- External ontology
- mediadive.medium:J21 (source link unavailable) — LACTOBACILLUS MEDIUM III
- Applications
-
- Microbial cultivation
- pH
- 5.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=21
Composition
flowchart LR
CultureMech_002581["lactobacillus_medium_iii"]
ing0_Casein_peptone["Casein peptone"]
CultureMech_002581 --> ing0_Casein_peptone
ing1_Yeast_extract["Yeast extract"]
CultureMech_002581 --> ing1_Yeast_extract
ing2_Beef_extract["Beef extract"]
CultureMech_002581 --> ing2_Beef_extract
ing3_Glucose["Glucose"]
CultureMech_002581 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_KH2PO4["KH2PO4"]
CultureMech_002581 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_K2HPO4["K2HPO4"]
CultureMech_002581 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002581 --> ing6_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002581 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_002581 --> ing8_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing8_MnSO4_x_n_H2O -.-> CHEBI_86360
ing9_Tween_80["Tween 80"]
CultureMech_002581 --> ing9_Tween_80
CHEBI_53426["CHEBI:53426"]
ing9_Tween_80 -.-> CHEBI_53426
ing10_Ethanol["Ethanol"]
CultureMech_002581 --> ing10_Ethanol
CHEBI_16236["CHEBI:16236"]
ing10_Ethanol -.-> CHEBI_16236
ing11_Sodium_acetate["Sodium acetate"]
CultureMech_002581 --> ing11_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing11_Sodium_acetate -.-> CHEBI_32954
ing12_DL_mevalonic_acid["DL-mevalonic acid"]
CultureMech_002581 --> ing12_DL_mevalonic_acid
CHEBI_25351["CHEBI:25351"]
ing12_DL_mevalonic_acid -.-> CHEBI_25351
ing13_Agar["Agar"]
CultureMech_002581 --> ing13_Agar
CHEBI_2509["CHEBI:2509"]
ing13_Agar -.-> CHEBI_2509
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casein peptone | 9.60615 G_PER_L | ( tryptic digest) | |
| 2 | Yeast extract | 4.80307 G_PER_L | ||
| 3 | Beef extract | 1.92123 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
19.2123 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.480307 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.480307 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.00960615 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.192123 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.00720461 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 10 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
|
| 11 |
Ethanol
C2H6O 46.069 g/mol
|
40 G_PER_L |
CHEBI:16236
ethanol
|
|
| 12 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
19.2123 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 13 |
DL-mevalonic acid
C6H12O4 148.158 g/mol
|
0.0288184 G_PER_L |
CHEBI:25351
mevalonic acid acid
|
|
| 14 | Agar | 14.4092 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 5.2.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J21_LACTOBACILLUS_MEDIUM_III (CultureMech:002581)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.596338Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J21 |
| 2026-03-13T06:19:14.307649Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002581 |
| 2026-03-17T06:30:11.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.536197+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LACTOBACILLUS MEDIUM III" to "lactobacillus_medium_iii" for consistent matching |
| 2026-06-05T07:40:16.853788+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.841163+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:35.817105+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 4f5278b7218311a0ae3c5c8066367e1c1c86298ce39d4910d00cbd784b4a4481 |