Overview
- External ontology
- mediadive.medium:J223 (source link unavailable) — JCM MEDIUM No. 223
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=223
Composition
flowchart LR
CultureMech_002585["jcm_medium_no_223"]
ing0_KH2PO4["KH2PO4"]
CultureMech_002585 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
CultureMech_002585 --> ing1_Na2HPO4_x_12_H2O
CHEBI_34683["CHEBI:34683"]
ing1_Na2HPO4_x_12_H2O -.-> CHEBI_34683
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002585 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_Yeast_extract["Yeast extract"]
CultureMech_002585 --> ing3_Yeast_extract
ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002585 --> ing4_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing4_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing5_Skim_milk["Skim milk"]
CultureMech_002585 --> ing5_Skim_milk
ing6_Resazurin["Resazurin"]
CultureMech_002585 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_Agar["Agar"]
CultureMech_002585 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.4985 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
|
4.1958 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.37962 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 | Yeast extract | 0.999001 G_PER_L | ||
| 5 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.0999001 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 6 | Skim milk | 9.99001 G_PER_L | ||
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 | Agar | 24.975 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Adjust pH to 7.5.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- jcm_medium_no_223 (CultureMech:002585)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.006483Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J223 |
| 2026-03-13T06:19:14.336116Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002585 |
| 2026-03-17T06:30:20.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.568952+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JCM MEDIUM No. 223" to "jcm_medium_no_223" for consistent matching |
| 2026-06-05T07:41:09.954692+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.236076+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:39.138351+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 54b2bf12300745a748e66dd713c747fad60249975843f58b1c4860062a0d1dc5 |