Overview
- External ontology
- mediadive.medium:J236 (source link unavailable) — THERMOCLADIUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 3.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=236
Composition
flowchart LR
CultureMech_002596["thermocladium_medium"]
ing0_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_002596 --> ing0_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing0_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing1_Yeast_extract["Yeast extract"]
CultureMech_002596 --> ing1_Yeast_extract
ing2_Resazurin["Resazurin"]
CultureMech_002596 --> ing2_Resazurin
CHEBI_8806["CHEBI:8806"]
ing2_Resazurin -.-> CHEBI_8806
ing3_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002596 --> ing3_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing3_Na2S_x_9_H2O -.-> CHEBI_76209
ing4_Sulfur["Sulfur"]
CultureMech_002596 --> ing4_Sulfur
CHEBI_26833["CHEBI:26833"]
ing4_Sulfur -.-> CHEBI_26833
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_002596 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_KH2PO4["KH2PO4"]
CultureMech_002596 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002596 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002596 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_002596 --> ing9_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing9_FeCl3_x_6_H2O -.-> CHEBI_86254
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002596 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
ing11_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_002596 --> ing11_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing11_Na2B4O7_x_10_H2O -.-> CHEBI_131366
ing12_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_002596 --> ing12_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing12_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002596 --> ing13_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_002596 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing15_VOSO4_x_n_H2O["VOSO4 x n H2O"]
CultureMech_002596 --> ing15_VOSO4_x_n_H2O
CHEBI_87020["CHEBI:87020"]
ing15_VOSO4_x_n_H2O -.-> CHEBI_87020
ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_002596 --> ing16_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
ing17_Biotin["Biotin"]
CultureMech_002596 --> ing17_Biotin
CHEBI_15956["CHEBI:15956"]
ing17_Biotin -.-> CHEBI_15956
ing18_Folic_acid["Folic acid"]
CultureMech_002596 --> ing18_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing18_Folic_acid -.-> CHEBI_27470
ing19_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002596 --> ing19_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing19_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing20_Thiamine_HCl["Thiamine HCl"]
CultureMech_002596 --> ing20_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing20_Thiamine_HCl -.-> CHEBI_49105
ing21_Riboflavin["Riboflavin"]
CultureMech_002596 --> ing21_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing21_Riboflavin -.-> CHEBI_17015
ing22_Nicotinic_acid["Nicotinic acid"]
CultureMech_002596 --> ing22_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing22_Nicotinic_acid -.-> CHEBI_15940
ing23_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002596 --> ing23_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing23_Calcium_pantothenate -.-> CHEBI_31345
ing24_Vitamin_B12["Vitamin B12"]
CultureMech_002596 --> ing24_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing24_Vitamin_B12 -.-> CHEBI_176843
ing25_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002596 --> ing25_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing25_p_Aminobenzoic_acid -.-> CHEBI_30753
ing26_Lipoic_acid["Lipoic acid"]
CultureMech_002596 --> ing26_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing26_Lipoic_acid -.-> CHEBI_16494
Ingredients (27)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
2.91089 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 2 | Yeast extract | 0.49505 G_PER_L | ||
| 3 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000990099 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 4 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.49505 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 5 |
Sulfur
S 32.067 g/mol
|
9.90099 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.07 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.002 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0018 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 12 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.0045 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
|
| 13 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.00022 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 14 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
5e-05 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
3e-05 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 16 |
VOSO4 x n H2O
(H2O)n.O5SV 181.021 g/mol
|
3e-05 G_PER_L |
CHEBI:87020
vanadyl sulfate hydrate
|
|
| 17 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
1e-05 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 18 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 19 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 20 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 21 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 22 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 23 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 24 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 25 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 26 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 27 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Combine Modified Brock's salt base solution, trisodium citrate and resazurin, and adjust pH to 3.5 with 10 N H2SO4. Autoclave under a N2 atmosphere. Separately autoclave yeast extract and Na2S x 9H2O as 10% and 5% solutions, respectively, under a N2 atmosphere. Filter-sterilize Trace vitamins. Steam sulfur for 3 hr on each of 3 successive days. Aseptically mix components except Na2S x 9H2O in suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) while gassing with H2-CO2 (80:20) gas mixture and seal with butyl rubber stoppers. Prior to inoculation, aseptically and anaerobically add the Na2S x 9H2O solutions and adjust pH to 4.0-4.5, if necessary. When the indicator (resazurin) turns to colorless, inoculate the culture and pressurize the vessels to 100 kPa H2-CO2 (80:20).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- thermocladium_medium (CultureMech:002596)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.045185Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J236 |
| 2026-01-28T19:48:20.288527Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:14.430617Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002596 |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.696144+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "THERMOCLADIUM MEDIUM" to "thermocladium_medium" for consistent matching |
| 2026-06-05T07:41:49.806420+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:12.871330+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:32.914451+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 87713397d350a522385d0402bc6134180f84e0c570b729143ab72bbbc9d52fe9 |