jcm_medium_no_244

CultureMech:002605 bacterial COMPLEX LIQUID
Original name: JCM MEDIUM No. 244

Overview

External ontology
mediadive.medium:J244 (source link unavailable) — JCM MEDIUM No. 244
Applications
  • Microbial cultivation
pH
6.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=244

Composition

flowchart LR
    CultureMech_002605["jcm_medium_no_244"]
    ing0_NaHCO3["NaHCO3"]
    CultureMech_002605 --> ing0_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing0_NaHCO3 -.-> CHEBI_32139
    ing1_KH2PO4["KH2PO4"]
    CultureMech_002605 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_Bacto_peptone["Bacto peptone"]
    CultureMech_002605 --> ing2_Bacto_peptone
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_002605 --> ing3_Yeast_extract
    ing4_Resazurin["Resazurin"]
    CultureMech_002605 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002605 --> ing5_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing5_Na2S_x_9_H2O -.-> CHEBI_76209
    ing6_Sulfur["Sulfur"]
    CultureMech_002605 --> ing6_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing6_Sulfur -.-> CHEBI_26833
    ing7_NaCl["NaCl"]
    CultureMech_002605 --> ing7_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing7_NaCl -.-> CHEBI_26710
    ing8_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002605 --> ing8_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing8_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002605 --> ing9_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing10_KCl["KCl"]
    CultureMech_002605 --> ing10_KCl
    CHEBI_32588["CHEBI:32588"]
    ing10_KCl -.-> CHEBI_32588
    ing11_NaBr["NaBr"]
    CultureMech_002605 --> ing11_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing11_NaBr -.-> CHEBI_63004
    ing12_H3BO3["H3BO3"]
    CultureMech_002605 --> ing12_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing12_H3BO3 -.-> CHEBI_33118
    ing13_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_002605 --> ing13_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing13_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing14_KI["KI"]
    CultureMech_002605 --> ing14_KI
    CHEBI_8346["CHEBI:8346"]
    ing14_KI -.-> CHEBI_8346
    ing15_Trisodium_citrate["Trisodium citrate"]
    CultureMech_002605 --> ing15_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing15_Trisodium_citrate -.-> CHEBI_53258
    ing16_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002605 --> ing16_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing16_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002605 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_002605 --> ing18__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing18__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing19_Na2SeO3["Na2SeO3"]
    CultureMech_002605 --> ing19_Na2SeO3
    CHEBI_48843["CHEBI:48843"]
    ing19_Na2SeO3 -.-> CHEBI_48843
    ing20_Na2SeO4["Na2SeO4"]
    CultureMech_002605 --> ing20_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing20_Na2SeO4 -.-> CHEBI_77775
    ing21_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002605 --> ing21_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing21_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing22_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002605 --> ing22_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing22_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing23_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002605 --> ing23_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing23_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing24_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002605 --> ing24_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing24_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing25_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002605 --> ing25_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing25_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing26_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002605 --> ing26_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing26_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing27_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002605 --> ing27_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing27_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing28_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002605 --> ing28_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing28_AlK_SO4_2 -.-> CHEBI_86463
    ing29_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002605 --> ing29_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing29_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (30)

# Ingredient Concentration Ontology Notes
1 NaHCO3
CHO3.Na 84.006 g/mol
0.156098 G_PER_L CHEBI:32139
sodium hydrogencarbonate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.487805 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 Bacto peptone 1.95122 G_PER_L
4 Yeast extract 0.97561 G_PER_L
5 Resazurin
C12H7NO4 229.191 g/mol
0.000731707 G_PER_L CHEBI:8806
Resazurin
6 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.487805 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
7 Sulfur
S 32.067 g/mol
9.7561 G_PER_L CHEBI:26833
sulfur atom
8 NaCl
Cl.Na 58.443 g/mol
56.4 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 55.4, 1.0]
9 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
17.0 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 14.0, 3.0]
10 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
11 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
11 KCl
Cl.K 74.551 g/mol
1.3 G_PER_L CHEBI:32588
potassium chloride
12 NaBr
Br.Na 102.894 g/mol
0.2 G_PER_L CHEBI:63004
sodium bromide
13 H3BO3
H3BO3 61.833 g/mol
0.06999999999999999 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.06, 0.01]
14 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.03 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
15 KI
I.K 166.002 g/mol
0.0001 G_PER_L CHEBI:8346
potassium iodide
16 Trisodium citrate
C6H5O7.3Na 258.069 g/mol
0.02 G_PER_L CHEBI:53258
sodium citrate
17 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.6 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 1.5, 0.1]
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.025 G_PER_L CHEBI:34887
nickel dichloride
19 (NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
2 G_PER_L CHEBI:86149
ammonium nickel sulfate hexahydrate
20 Na2SeO3
2Na.O3Se 172.937 g/mol
0.0003 G_PER_L CHEBI:48843
disodium selenite
21 Na2SeO4
2Na.O4Se 188.936 g/mol
0.01 G_PER_L CHEBI:77775
sodium selenate
22 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.01 G_PER_L CHEBI:63939
sodium tungstate dihydrate
23 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
24 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
25 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
26 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
27 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
28 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
29 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
30 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix ingredients, except sulfur and Na2S x 9H2O, and adjust pH to 6.0 with H2SO4. Sterilize the medium by filtration. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S x 9H2O as a 5% solution, and autoclave under a N2 atmosphere. Distribute the medium and sulfur into culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Prior to inoculation, add the sterile Na2S x 9H2O solution, and readjust pH to 6.0 with sterile 1 N H2SO4. Pressurize the inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
  2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:16.073500Z mediadive-import Imported from MediaDive Source: JCM, ID: J244
2026-03-13T03:08:54.552023Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:14.507958Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002605
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.780696+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "JCM MEDIUM No. 244" to "jcm_medium_no_244" for consistent matching
2026-06-05T07:41:10.010809+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.016969+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:00.254823+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:39.189154+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 0387c02c09d6e344ec3f2d805eeb937d243448425c63cab1ab5e514a1490dd47