mj_ypgs_medium

CultureMech:002625 bacterial COMPLEX LIQUID
Original name: MJ/YPGS MEDIUM

Overview

External ontology
mediadive.medium:J268 (source link unavailable) — MJ/YPGS MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=268

Composition

flowchart LR
    CultureMech_002625["mj_ypgs_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_002625 --> ing0_Yeast_extract
    ing1_Bacto_peptone["Bacto peptone"]
    CultureMech_002625 --> ing1_Bacto_peptone
    ing2_Glucose["Glucose"]
    CultureMech_002625 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Sodium_succinate["Sodium succinate"]
    CultureMech_002625 --> ing3_Sodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing3_Sodium_succinate -.-> CHEBI_63675
    ing4_Na2SiO3_x_9_H2O["Na2SiO3 x 9 H2O"]
    CultureMech_002625 --> ing4_Na2SiO3_x_9_H2O
    CHEBI_132108["CHEBI:132108"]
    ing4_Na2SiO3_x_9_H2O -.-> CHEBI_132108
    ing5_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_002625 --> ing5_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing5_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing6_NaCl["NaCl"]
    CultureMech_002625 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710
    ing7_K2HPO4["K2HPO4"]
    CultureMech_002625 --> ing7_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing7_K2HPO4 -.-> CHEBI_131527
    ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002625 --> ing8_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002625 --> ing9_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing10_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002625 --> ing10_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing10_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing11_KCl["KCl"]
    CultureMech_002625 --> ing11_KCl
    CHEBI_32588["CHEBI:32588"]
    ing11_KCl -.-> CHEBI_32588
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002625 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_002625 --> ing13_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing13_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing14_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
    CultureMech_002625 --> ing14_Fe_NH4_2_SO4_2_x_6_H2O
    CHEBI_76181["CHEBI:76181"]
    ing14_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
    ing15_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002625 --> ing15_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing15_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing16_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002625 --> ing16_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing16_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing17_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002625 --> ing17_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing17_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing18_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002625 --> ing18_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing18_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing19_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002625 --> ing19_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing19_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing20_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002625 --> ing20_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing20_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing21_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002625 --> ing21_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing21_AlK_SO4_2 -.-> CHEBI_86463
    ing22_H3BO3["H3BO3"]
    CultureMech_002625 --> ing22_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing22_H3BO3 -.-> CHEBI_33118
    ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002625 --> ing23_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (24)

# Ingredient Concentration Ontology Notes
1 Yeast extract 1 G_PER_L
2 Bacto peptone 1 G_PER_L
3 Glucose
C6H12O6 180.156 g/mol
0.2 G_PER_L CHEBI:17234
glucose
4 Sodium succinate
C4H4O4.2Na 162.052 g/mol
0.2 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
5 Na2SiO3 x 9 H2O
9H2O.2Na.O3Si 284.198 g/mol
1 G_PER_L CHEBI:132108
sodium silicate nonahydrate
6 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
2.48 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
7 NaCl
Cl.Na 58.443 g/mol
30.703 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 29.703, 1.0]
8 K2HPO4
HO4P.2K 174.174 g/mol
0.138614 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
9 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.238614 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.138614, 0.1]
10 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
6.36634 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 3.36634, 3.0]
11 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
4.13861 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
12 KCl
Cl.K 74.551 g/mol
0.326733 G_PER_L CHEBI:32588
potassium chloride
13 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.00049505 G_PER_L CHEBI:34887
nickel dichloride
14 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.00049505 G_PER_L CHEBI:131361
disodium selenite pentahydrate
15 Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
0.00990099 G_PER_L CHEBI:76181
ferrous ammonium sulfate hexahydrate
16 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
17 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
18 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
19 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
20 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
21 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
22 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
23 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
24 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust pH to 7.5.
  2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:16.131826Z mediadive-import Imported from MediaDive Source: JCM, ID: J268
2026-01-28T19:48:42.774409Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T03:09:43.936694Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:14.615624Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002625
2026-03-17T06:30:56.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.900374+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MJ/YPGS MEDIUM" to "mj_ypgs_medium" for consistent matching
2026-06-05T07:41:35.033745+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.157063+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:46.688734+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 1b646760cb6e3cb8dacfb6b6c7bb06fec11d739771fe3d2b8b8995f6de416bcf