jcm_medium_no_272

Overview

External ontology

mediadive.medium:J272 (source link unavailable) — JCM MEDIUM No. 272

Applications

• Microbial cultivation

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=272

Composition

flowchart LR
    CultureMech_002629["jcm_medium_no_272"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_002629 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_NH4Cl["NH4Cl"]
    CultureMech_002629 --> ing1_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing1_NH4Cl -.-> CHEBI_31206
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002629 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_NaCl["NaCl"]
    CultureMech_002629 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_Sodium_acetate["Sodium acetate"]
    CultureMech_002629 --> ing4_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing4_Sodium_acetate -.-> CHEBI_32954
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_002629 --> ing5_Yeast_extract
    ing6_Trypticase_peptone["Trypticase peptone"]
    CultureMech_002629 --> ing6_Trypticase_peptone
    ing7_Na2CO3["Na2CO3"]
    CultureMech_002629 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_002629 --> ing8_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_002629 --> ing9_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing9_Na2S_x_9_H2O -.-> CHEBI_76209
    ing10_Resazurin["Resazurin"]
    CultureMech_002629 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_Biotin["Biotin"]
    CultureMech_002629 --> ing11_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing11_Biotin -.-> CHEBI_15956
    ing12_Folic_acid["Folic acid"]
    CultureMech_002629 --> ing12_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing12_Folic_acid -.-> CHEBI_27470
    ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_002629 --> ing13_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing14_Thiamine_HCl["Thiamine HCl"]
    CultureMech_002629 --> ing14_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing14_Thiamine_HCl -.-> CHEBI_49105
    ing15_Riboflavin["Riboflavin"]
    CultureMech_002629 --> ing15_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing15_Riboflavin -.-> CHEBI_17015
    ing16_Nicotinic_acid["Nicotinic acid"]
    CultureMech_002629 --> ing16_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing16_Nicotinic_acid -.-> CHEBI_15940
    ing17_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_002629 --> ing17_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing17_Calcium_pantothenate -.-> CHEBI_31345
    ing18_Vitamin_B12["Vitamin B12"]
    CultureMech_002629 --> ing18_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing18_Vitamin_B12 -.-> CHEBI_176843
    ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_002629 --> ing19_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing20_Lipoic_acid["Lipoic acid"]
    CultureMech_002629 --> ing20_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing20_Lipoic_acid -.-> CHEBI_16494
    ing21_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002629 --> ing21_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing21_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing22_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_002629 --> ing22_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing22_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing23_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002629 --> ing23_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing23_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing24_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002629 --> ing24_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing24_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing25_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002629 --> ing25_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing25_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing26_ZnCl2["ZnCl2"]
    CultureMech_002629 --> ing26_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing26_ZnCl2 -.-> CHEBI_49976
    ing27_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002629 --> ing27_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing27_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing28_H3BO3["H3BO3"]
    CultureMech_002629 --> ing28_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing28_H3BO3 -.-> CHEBI_33118
    ing29_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002629 --> ing29_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing29_NiCl2_x_6_H2O -.-> CHEBI_34887
    CultureMech_002629 --> more["...1 more"]

Ingredients (31)

Preparation

1. Mix ingredients, except Na2CO3, L-cysteine x HCl x H2O and Na2S x 9H2O. Bring to a boil for 5-10 sec, then, cool down under a stream of H2-CO2 (80:20, v/v) and add Na2CO3. Dispense the medium into suitable culture vessels (e.g., 20 ml of the medium in 120 ml serum bottles) under a stream of H2-CO2 (80:20, v/v) and seal with butyl rubber stoppers. After autoclaving, stand the medium overnight. Separately autoclave cysteine x HCl and Na2S x 9H2O as 5% solutions, respectively, under a N2 atmosphere. Prior to inoculation, aseptically and anaerobically add the L-cysteine x HCl x H2O and Na2S x 9H2O solutions. Check pH of the medium to be around 7.5. Pressurize the inoculated bottles to 200 kPa H2-CO2 (80:20, v/v).
2. Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH to 6.5 with KOH. Then add the remaining salts and adjust volume to 1.0 L. Readjust pH to 7.0 with KOH.
3. Comment: For strain JCM 10134, incubate with a 10-20% inoculum.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• jcm_medium_no_272 (CultureMech:002629)