Overview
- External ontology
- mediadive.medium:J277 (source link unavailable) — MJYP* MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=277
Composition
flowchart LR
CultureMech_002634["mjyp_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_002634 --> ing0_Yeast_extract
ing1_Trypticase_peptone["Trypticase peptone"]
CultureMech_002634 --> ing1_Trypticase_peptone
ing2_NaNO3["NaNO3"]
CultureMech_002634 --> ing2_NaNO3
CHEBI_63005["CHEBI:63005"]
ing2_NaNO3 -.-> CHEBI_63005
ing3_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_002634 --> ing3_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing3_FeSO4_x_7_H2O -.-> CHEBI_75836
ing4_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_002634 --> ing4_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing4_Na2S_x_9_H2O -.-> CHEBI_76209
ing5_Resazurin["Resazurin"]
CultureMech_002634 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_NaCl["NaCl"]
CultureMech_002634 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_K2HPO4["K2HPO4"]
CultureMech_002634 --> ing7_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing7_K2HPO4 -.-> CHEBI_131527
ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002634 --> ing8_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_002634 --> ing9_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
ing10_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_002634 --> ing10_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing10_MgCl2_x_6_H2O -.-> CHEBI_86345
ing11_KCl["KCl"]
CultureMech_002634 --> ing11_KCl
CHEBI_32588["CHEBI:32588"]
ing11_KCl -.-> CHEBI_32588
ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002634 --> ing12_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
ing13_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_002634 --> ing13_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing13_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing14_Fe_NH4_2_SO4_2_x_6_H2O["Fe(NH4)2(SO4)2 x 6 H2O"]
CultureMech_002634 --> ing14_Fe_NH4_2_SO4_2_x_6_H2O
CHEBI_76181["CHEBI:76181"]
ing14_Fe_NH4_2_SO4_2_x_6_H2O -.-> CHEBI_76181
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 2 G_PER_L | ||
| 2 | Trypticase peptone | 2 G_PER_L | ||
| 3 |
NaNO3
NO3.Na 84.994 g/mol
|
1.7 G_PER_L |
CHEBI:63005
sodium nitrate
|
|
| 4 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.39 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 5 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
29.703 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.138614 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 9 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.138614 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 10 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.36634 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 11 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.13861 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 12 |
KCl
Cl.K 74.551 g/mol
|
0.326733 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 13 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.00049505 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 14 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.00049505 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 15 |
Fe(NH4)2(SO4)2 x 6 H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.00990099 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
Preparation
- Mix ingredients, except NaNO3, FeSO4 x 7H2O and Na2S x 9H2O, and autoclave under a N2 atmosphere. Separately autoclave NaNO3, FeSO4 x 7H2O and Na2S x 9H2O solutions (pH 7.0, 7.5 and 7.5, respectively) under a N2 atmosphere. Aseptically and anaerobically add the NaNO3, FeSO4 x 7H2O and Na2S x 9H2O solutions to the medium and adjust pH to 6.0. Pressurize the inoculated bottle to 200 kPa N2.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mjyp_medium (CultureMech:002634)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.157839Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J277 |
| 2026-01-28T19:48:09.454610Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:14.680832Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002634 |
| 2026-03-17T06:29:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:27.973461+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MJYP* MEDIUM" to "mjyp_medium" for consistent matching |
| 2026-06-05T07:41:35.140551+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.197951+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:46.796398+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint ae2e6d8a8b684c9732b6b2888c1d872d500046fc4dcc61499ff79df5fa42197a |