hydrogenophilus_hirschii_medium

CultureMech:002636 bacterial DEFINED LIQUID
Original name: HYDROGENOPHILUS HIRSCHII MEDIUM

Overview

External ontology
mediadive.medium:J279 (source link unavailable) — HYDROGENOPHILUS HIRSCHII MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=279

Composition

flowchart LR
    CultureMech_002636["hydrogenophilus_hirschii_medium"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002636 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_002636 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_KCl["KCl"]
    CultureMech_002636 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_NaBr["NaBr"]
    CultureMech_002636 --> ing3_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing3_NaBr -.-> CHEBI_63004
    ing4_NaHCO3["NaHCO3"]
    CultureMech_002636 --> ing4_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing4_NaHCO3 -.-> CHEBI_32139
    ing5_NH4Cl["NH4Cl"]
    CultureMech_002636 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002636 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_002636 --> ing7__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing7__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing8_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002636 --> ing8_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing8_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing9_Na2SeO4["Na2SeO4"]
    CultureMech_002636 --> ing9_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing9_Na2SeO4 -.-> CHEBI_77775
    ing10_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_002636 --> ing10_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing10_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_002636 --> ing11_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing12_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_002636 --> ing12_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing12_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing13_NaCl["NaCl"]
    CultureMech_002636 --> ing13_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing13_NaCl -.-> CHEBI_26710
    ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_002636 --> ing14_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_002636 --> ing15_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_002636 --> ing16_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_002636 --> ing17_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing18_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_002636 --> ing18_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing18_AlK_SO4_2 -.-> CHEBI_86463
    ing19_H3BO3["H3BO3"]
    CultureMech_002636 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002636 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
9.93069 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 6.93069, 3.0]
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
5.44554 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 KCl
Cl.K 74.551 g/mol
0.643564 G_PER_L CHEBI:32588
potassium chloride
4 NaBr
Br.Na 102.894 g/mol
0.0990099 G_PER_L CHEBI:63004
sodium bromide
5 NaHCO3
CHO3.Na 84.006 g/mol
1.9802 G_PER_L CHEBI:32139
sodium hydrogencarbonate
6 NH4Cl
Cl.H4N 53.492 g/mol
0.148515 G_PER_L CHEBI:31206
ammonium chloride
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.59505 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.49505, 0.1]
8 (NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
0.019802 G_PER_L CHEBI:86149
ammonium nickel sulfate hexahydrate
9 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
9.90099e-05 G_PER_L CHEBI:63939
sodium tungstate dihydrate
10 Na2SeO4
2Na.O4Se 188.936 g/mol
9.90099e-05 G_PER_L CHEBI:77775
sodium selenate
11 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
0.990099 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
12 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
13 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
14 NaCl
Cl.Na 58.443 g/mol
1 G_PER_L CHEBI:26710
sodium chloride
15 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
16 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
17 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
18 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
19 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
20 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Adjust pH to 7.0 with H2SO4. Distribute 20 ml of medium into 120 ml serum bottles under a stream of a H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Autoclave and stand the bottles overnight. Add 15 ml filter-sterilized air and pressurize the gas phase to 200 kPa with a H2-CO2 (4:1, v/v) gas mixture.
  2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:16.161943Z mediadive-import Imported from MediaDive Source: JCM, ID: J279
2026-03-13T03:09:33.135301Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:14.692724Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002636
2026-03-17T06:30:43.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:27.986325+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HYDROGENOPHILUS HIRSCHII MEDIUM" to "hydrogenophilus_hirschii_medium" for consistent matching
2026-06-05T07:41:09.068541+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.979145+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:59.939344+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:38.189236+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 62c1e37a264350da983c5542ecdf6a5572b6c2006e9b09372a8fc19f6a37299d