halorhabdus_medium

CultureMech:002651 archaea COMPLEX LIQUID
Original name: HALORHABDUS MEDIUM

Overview

External ontology
mediadive.medium:J294 (source link unavailable) — HALORHABDUS MEDIUM
Applications
  • Microbial cultivation
pH
7.0
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=294

Composition

flowchart LR
    CultureMech_002651["halorhabdus_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_002651 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_NaBr["NaBr"]
    CultureMech_002651 --> ing1_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing1_NaBr -.-> CHEBI_63004
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_002651 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_002651 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NH4Cl["NH4Cl"]
    CultureMech_002651 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Tris_HCl_buffer["Tris-HCl buffer"]
    CultureMech_002651 --> ing5_Tris_HCl_buffer
    CHEBI_9754["CHEBI:9754"]
    ing5_Tris_HCl_buffer -.-> CHEBI_9754
    ing6_Yeast_extract["Yeast extract"]
    CultureMech_002651 --> ing6_Yeast_extract
    ing7_Glucose["Glucose"]
    CultureMech_002651 --> ing7_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing7_Glucose -.-> CHEBI_17234
    ing8_KH2PO4["KH2PO4"]
    CultureMech_002651 --> ing8_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing8_KH2PO4 -.-> CHEBI_63036
    ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_002651 --> ing9_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing10_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_002651 --> ing10_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing10_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_002651 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_HCl["HCl"]
    CultureMech_002651 --> ing12_HCl
    CHEBI_17883["CHEBI:17883"]
    ing12_HCl -.-> CHEBI_17883
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_002651 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_ZnCl2["ZnCl2"]
    CultureMech_002651 --> ing14_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing14_ZnCl2 -.-> CHEBI_49976
    ing15_H3BO3["H3BO3"]
    CultureMech_002651 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_002651 --> ing16_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing17_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_002651 --> ing17_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing17_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing18_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_002651 --> ing18_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing18_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing19_AlCl3["AlCl3"]
    CultureMech_002651 --> ing19_AlCl3
    CHEBI_30114["CHEBI:30114"]
    ing19_AlCl3 -.-> CHEBI_30114
    ing20_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_002651 --> ing20_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing20_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
270 G_PER_L CHEBI:26710
sodium chloride
2 NaBr
Br.Na 102.894 g/mol
0.1 G_PER_L CHEBI:63004
sodium bromide
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
20 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 KCl
Cl.K 74.551 g/mol
5 G_PER_L CHEBI:32588
potassium chloride
5 NH4Cl
Cl.H4N 53.492 g/mol
2 G_PER_L CHEBI:31206
ammonium chloride
6 Tris-HCl buffer
C4H11NO3 121.136 g/mol
12 G_PER_L CHEBI:9754
tris
7 Yeast extract 1 G_PER_L
8 Glucose
C6H12O6 180.156 g/mol
2 G_PER_L CHEBI:17234
glucose
9 KH2PO4
H2O4P.K 136.084 g/mol
0.125 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
10 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
11 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.9851999999999999 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
 [Merged 2 duplicates: 0.005, 1.9802]
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1040099 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 [Merged 2 duplicates: 0.005, 0.0990099]
13 HCl
HCl 36.461 g/mol
3.16832 G_PER_L CHEBI:17883
hydrogen chloride
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.247525 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 ZnCl2
2Cl.Zn 136.296 g/mol
0.0693069 G_PER_L CHEBI:49976
zinc dichloride
16 H3BO3
H3BO3 61.833 g/mol
0.00594059 G_PER_L CHEBI:33118
boric acid
17 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.039604 G_PER_L CHEBI:75213
sodium molybdate dihydrate
18 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.0693069 G_PER_L CHEBI:34887
nickel dichloride
19 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.0019802 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
20 AlCl3
AlCl3 133.341 g/mol
0.0247525 G_PER_L CHEBI:30114
aluminium trichloride
21 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.00594059 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Add components except yeast extract to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 with NaOH and sterilize by filtration. Autoclave yeast extract as a 10% (w/v) solution and aseptically add to the mineral solution.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:16.205254Z mediadive-import Imported from MediaDive Source: JCM, ID: J294
2026-03-13T03:09:12.220784Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:14.780225Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:002651
2026-03-17T06:30:19.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:28.083739+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALORHABDUS MEDIUM" to "halorhabdus_medium" for consistent matching
2026-06-05T07:41:08.100988+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:59.629294+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:28.895272+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint ccf58705821e62e8e70efd2d8e790adfe9a6bab96f7c471cfd7bc98c392b0551