Overview
- External ontology
- mediadive.medium:J335 (source link unavailable) — JCM MEDIUM No. 335
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=335
Composition
flowchart LR
CultureMech_002694["jcm_medium_no_335"]
ing0_K2HPO4["K2HPO4"]
CultureMech_002694 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_KH2PO4["KH2PO4"]
CultureMech_002694 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_Na3_EDTA["Na3-EDTA"]
CultureMech_002694 --> ing2_Na3_EDTA
CHEBI_63125["CHEBI:63125"]
ing2_Na3_EDTA -.-> CHEBI_63125
ing3_FeSO4_x_H2O["FeSO4 x H2O"]
CultureMech_002694 --> ing3_FeSO4_x_H2O
CHEBI_75834["CHEBI:75834"]
ing3_FeSO4_x_H2O -.-> CHEBI_75834
ing4_MgSO4_x_H2O["MgSO4 x H2O"]
CultureMech_002694 --> ing4_MgSO4_x_H2O
CHEBI_32599["CHEBI:32599"]
ing4_MgSO4_x_H2O -.-> CHEBI_32599
ing5_CaCl2_x_H2O["CaCl2 x H2O"]
CultureMech_002694 --> ing5_CaCl2_x_H2O
CHEBI_3312["CHEBI:3312"]
ing5_CaCl2_x_H2O -.-> CHEBI_3312
ing6_NaCl["NaCl"]
CultureMech_002694 --> ing6_NaCl
CHEBI_26710["CHEBI:26710"]
ing6_NaCl -.-> CHEBI_26710
ing7_NH4Cl["NH4Cl"]
CultureMech_002694 --> ing7_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing7_NH4Cl -.-> CHEBI_31206
ing8_Na2SO4["Na2SO4"]
CultureMech_002694 --> ing8_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing8_Na2SO4 -.-> CHEBI_32149
ing9_Sodium_acetate["Sodium acetate"]
CultureMech_002694 --> ing9_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing9_Sodium_acetate -.-> CHEBI_32954
ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_002694 --> ing10_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing11_Biotin["Biotin"]
CultureMech_002694 --> ing11_Biotin
CHEBI_15956["CHEBI:15956"]
ing11_Biotin -.-> CHEBI_15956
ing12_Folic_acid["Folic acid"]
CultureMech_002694 --> ing12_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing12_Folic_acid -.-> CHEBI_27470
ing13_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_002694 --> ing13_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing13_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing14_Thiamine_HCl["Thiamine HCl"]
CultureMech_002694 --> ing14_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing14_Thiamine_HCl -.-> CHEBI_49105
ing15_Riboflavin["Riboflavin"]
CultureMech_002694 --> ing15_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing15_Riboflavin -.-> CHEBI_17015
ing16_Nicotinic_acid["Nicotinic acid"]
CultureMech_002694 --> ing16_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing16_Nicotinic_acid -.-> CHEBI_15940
ing17_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_002694 --> ing17_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing17_Calcium_pantothenate -.-> CHEBI_31345
ing18_Vitamin_B12["Vitamin B12"]
CultureMech_002694 --> ing18_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing18_Vitamin_B12 -.-> CHEBI_176843
ing19_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_002694 --> ing19_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing19_p_Aminobenzoic_acid -.-> CHEBI_30753
ing20_Lipoic_acid["Lipoic acid"]
CultureMech_002694 --> ing20_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing20_Lipoic_acid -.-> CHEBI_16494
ing21_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_002694 --> ing21_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing21_Nitrilotriacetic_acid -.-> CHEBI_44557
ing22_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_002694 --> ing22_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing22_FeCl3_x_6_H2O -.-> CHEBI_86254
ing23_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_002694 --> ing23_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing23_MnCl2_x_4_H2O -.-> CHEBI_86368
ing24_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_002694 --> ing24_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing24_CoCl2_x_6_H2O -.-> CHEBI_53503
ing25_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_002694 --> ing25_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing25_CaCl2_x_2_H2O -.-> CHEBI_86158
ing26_ZnCl2["ZnCl2"]
CultureMech_002694 --> ing26_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing26_ZnCl2 -.-> CHEBI_49976
ing27_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_002694 --> ing27_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing27_CuCl2_x_2_H2O -.-> CHEBI_86318
ing28_H3BO3["H3BO3"]
CultureMech_002694 --> ing28_H3BO3
CHEBI_33118["CHEBI:33118"]
ing28_H3BO3 -.-> CHEBI_33118
ing29_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_002694 --> ing29_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing29_NiCl2_x_6_H2O -.-> CHEBI_34887
CultureMech_002694 --> more["...1 more"]
Ingredients (31)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.764706 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.735294 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
Na3-EDTA
C10H13N2O8.3Na 358.19 g/mol
|
0.0392157 G_PER_L |
CHEBI:63125
EDTA trisodium salt
|
|
| 4 |
FeSO4 x H2O
Fe.H2O.O4S 169.923 g/mol
|
0.00980392 G_PER_L |
CHEBI:75834
iron(2+) sulfate monohydrate
|
|
| 5 |
MgSO4 x H2O
Mg.O4S 120.368 g/mol
|
0.245098 G_PER_L |
CHEBI:32599
magnesium sulfate
|
|
| 6 |
CaCl2 x H2O
Ca.2Cl 110.984 g/mol
|
0.0294118 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
1.196078 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 0.196078, 1.0] |
| 8 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.0264706 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 9 |
Na2SO4
2Na.O4S 142.043 g/mol
|
2.7451 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 10 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.156863 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 11 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.245098 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 12 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 13 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 14 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 15 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 16 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 17 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 18 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 19 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 20 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 21 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 22 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
12.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 23 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
1.35 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 24 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 25 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.024 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 26 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 27 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.1 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 28 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.025 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 29 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 30 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.12 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 31 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.026 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Preparation
- Adjust pH to 5.5. Fill the gas phase with H2-CO2 (80:20).
- Dissolve nitrilotriacetic acid first in 200 ml of distilled water, and adjust pH to 6.5 with KOH. Then add the remaining salts and adjust volume to 1.0 L. Readjust pH to 7.0 with KOH.
- Comment: For strain JCM 10134, incubate with a 10-20% inoculum.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- jcm_medium_no_335 (CultureMech:002694)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:16.321379Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J335 |
| 2026-03-13T03:09:05.443400Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:15.062975Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:002694 |
| 2026-03-17T06:30:11.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:28.404403+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "JCM MEDIUM No. 335" to "jcm_medium_no_335" for consistent matching |
| 2026-06-05T07:41:10.158845+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.308555+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:39.349386+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 8cfc480f89fe54fd90b087a9b47d4f37b40db4e9a23e05a1f45581799f028d14 |